Multifaceted role of nitric oxide in an in vitro mouse neuronal injury model: transcriptomic profiling defines the temporal recruitment of death signalling cascades

Peng, Zhao Feng, Chen, Minghui Jessica, Manikandan, Jayapal, Melendez, Alirio J., Shui, Guanghou, Russo-Marie, Françoise, Whiteman, Matthew, Beart, Philip M., Moore, Philip K. and Cheung, Nam Sang 2012, Multifaceted role of nitric oxide in an in vitro mouse neuronal injury model: transcriptomic profiling defines the temporal recruitment of death signalling cascades, Journal of cellular and molecular medicine, vol. 16, no. 1, pp. 41-58.

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Title Multifaceted role of nitric oxide in an in vitro mouse neuronal injury model: transcriptomic profiling defines the temporal recruitment of death signalling cascades
Author(s) Peng, Zhao Feng
Chen, Minghui Jessica
Manikandan, Jayapal
Melendez, Alirio J.
Shui, Guanghou
Russo-Marie, Françoise
Whiteman, Matthew
Beart, Philip M.
Moore, Philip K.
Cheung, Nam Sang
Journal name Journal of cellular and molecular medicine
Volume number 16
Issue number 1
Start page 41
End page 58
Total pages 18
Publisher Wiley-Blackwell Publishing
Place of publication Oxford, England
Publication date 2012-01
ISSN 1582-1838
1582-4934
Keyword(s) microarray
neuronal injury
nitric oxide
oxidative stress
reactive nitrogen species
reactive oxygen species
Summary Nitric oxide is implicated in the pathogenesis of various neuropathologies characterized by oxidative stress. Although nitric oxide has been reported to be involved in the exacerbation of oxidative stress observed in several neuropathologies, existent data fail to provide a holistic description of how nitrergic pathobiology elicits neuronal injury. Here we provide a comprehensive description of mechanisms contributing to nitric oxide induced neuronal injury by global transcriptomic profiling. Microarray analyses were undertaken on RNA from murine primary cortical neurons treated with the nitric oxide generator DETA-NONOate (NOC-18, 0.5 mM) for 8–24 hrs. Biological pathway analysis focused upon 3672 gene probes which demonstrated at least a ±1.5-fold expression in a minimum of one out of three time-points and passed statistical analysis (one-way anova, P < 0.05). Numerous enriched processes potentially determining nitric oxide mediated neuronal injury were identified from the transcriptomic profile: cell death, developmental growth and survival, cell cycle, calcium ion homeostasis, endoplasmic reticulum stress, oxidative stress, mitochondrial homeostasis, ubiquitin-mediated proteolysis, and GSH and nitric oxide metabolism. Our detailed time-course study of nitric oxide induced neuronal injury allowed us to provide the first time a holistic description of the temporal sequence of cellular events contributing to nitrergic injury. These data form a foundation for the development of screening platforms and define targets for intervention in nitric oxide neuropathologies where nitric oxide mediated injury is causative.
Language eng
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30046924

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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