Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS

Macindoe, Ingrid, Kwan, Ann H., Ren, Qin, Morris, Vanessa K., Yang, Wenrong, Mackay, Joel P. and Sunde, Margaret 2012, Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS, National Academy of Sciences. Proceedings, vol. 109, no. 14, pp. E804-E811.

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Title Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS
Author(s) Macindoe, Ingrid
Kwan, Ann H.
Ren, Qin
Morris, Vanessa K.
Yang, Wenrong
Mackay, Joel P.
Sunde, Margaret
Journal name National Academy of Sciences. Proceedings
Volume number 109
Issue number 14
Start page E804
End page E811
Total pages 8
Publisher National Academy of Sciences
Place of publication Washington, D. C.
Publication date 2012-04-03
ISSN 0027-8424
1091-6490
Keyword(s) fibril formation
proteins
surface
aggregation
sequence
nanostructures
prediction
mechanism
peptides
insights
Summary The hydrophobin EAS from the fungus Neurospora crassa forms functional amyloid fibrils called rodlets that facilitate spore formation and dispersal. Self-assembly of EAS into fibrillar rodlets occurs spontaneously at hydrophobic:hydrophilic interfaces and the rodlets further associate laterally to form amphipathic monolayers. We have used site-directed mutagenesis and peptide experiments to identify the region of EAS that drives intermolecular association and formation of the cross-β rodlet structure. Transplanting this region into a nonamyloidogenic hydrophobin enables it to form rodlets. We have also determined the structure and dynamics of an EAS variant with reduced rodlet-forming ability. Taken together, these data allow us to pinpoint the conformational changes that take place when hydrophobins self-assemble at an interface and to propose a model for the amphipathic EAS rodlet structure.
Language eng
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047006

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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