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Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii

Phoungpetchara, Ittipon, Tinikul, Yotsawan, Poljaroen, Jaruwan, Changklungmoa, Narin, Siangcham, Tanapan, Sroyraya, Norakot, Chotwiwatthanakun, Charoonroj, Vanichviriyakit, Rapeepun, Hanna, Peter J. and Sobhon, Prasert 2012, Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii, Cell and tissue research, vol. 348, no. 3, pp. 609-623.

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Title Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii
Formatted title Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii
Author(s) Phoungpetchara, Ittipon
Tinikul, Yotsawan
Poljaroen, Jaruwan
Changklungmoa, Narin
Siangcham, Tanapan
Sroyraya, Norakot
Chotwiwatthanakun, Charoonroj
Vanichviriyakit, Rapeepun
Hanna, Peter J.
Sobhon, Prasert
Journal name Cell and tissue research
Volume number 348
Issue number 3
Start page 609
End page 623
Total pages 15
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 2012
ISSN 0302-766X
1432-0878
Keyword(s) Macrobrachium rosenbergii
spermatic duct
histology
sperm
phosphorylated proteins
capacitation
Summary Phosphorylated sperm proteins are crucial for sperm maturation and capacitation as a priori to their fertilization with eggs. In the freshwater prawn, Macrobrachium rosenbergii, a male reproduction-related protein (Mar-Mrr) was known to be expressed only in the spermatic ducts as a protein with putative phosphorylation and may be involved in sperm capacitation in this species. We investigated further the temporal and spatial expression of the Mar-Mrr gene using RT-PCR and in situ hybridization and the characteristics and fate of the protein using immunblotting and immunocytochemistry. The Mar-Mrr gene was first expressed in 4-week-old post larvae and the protein was produced in epithelial cells lining the spermatic ducts, at the highest level in the proximal region and decreased in the middle and distal parts. The native protein had a MW of 17 kDa and a high degree of serine/threonine phosphorylation. It was transferred from the epithelial cells to become a major protein at the anterior region of the sperm. We suggest that it is involved in sperm capacitation and fertilization in this open thelycal species and this is being investigated.
Language eng
Field of Research 070401 Aquaculture
Socio Economic Objective 830103 Aquaculture Molluscs (excl. Oysters)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2012, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047072

Document type: Journal Article
Collection: Faculty of Science, Engineering and Built Environment
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Created: Mon, 13 Aug 2012, 13:12:30 EST

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