Physiological metal uptake by Nostoc punctiforme

Hudek, L., Rai, S., Michalczyk, A., Rai, L. C., Neilan, B. A. and Ackland, M. Leigh 2012, Physiological metal uptake by Nostoc punctiforme, BioMetals, vol. 25, pp. 893-903, doi: 10.1007/s10534-012-9556-4.

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Title Physiological metal uptake by Nostoc punctiforme
Formatted title Physiological metal uptake by Nostoc punctiforme
Author(s) Hudek, L.
Rai, S.
Michalczyk, A.ORCID iD for Michalczyk, A.
Rai, L. C.
Neilan, B. A.
Ackland, M. LeighORCID iD for Ackland, M. Leigh
Journal name BioMetals
Volume number 25
Start page 893
End page 903
Total pages 11
Publisher Springer
Place of publication Dordrecht, The Netherlands
Publication date 2012
ISSN 0966-0844
Keyword(s) absorption
divalent cations
metal uptake
Summary Trace metals are required for many cellular processes. The acquisition of trace elements from the environment includes a rapid adsorption of metals to the cell surface, followed by a slower internalization. We investigated the uptake of the trace elements Co2+, Cu2+, Mn2+, Ni2+, and Zn2+ and the non-essential divalent cation Cd2+ in the cyanobacterium Nostoc punctiforme. For each metal, a dose response study based on cell viability showed that the highest non-toxic concentrations were: 0.5 μM Cd2+, 2 μM Co2+, 0.5 μM Cu2+, 500 μM Mn2+, 1 μM Ni2+, and 18 μM Zn2+. Cells exposed to these non-toxic concentrations with combinations of Zn2+ and Cd2+, Zn2+ and Co2+, Zn2+ and Cu2+ or Zn2+ and Ni2+, had reduced growth in comparison to controls. Cells exposed to metal combinations with the addition of 500 μM Mn2+ showed similar growth compared to the untreated controls. Metal levels were measured after one and 72 h for whole cells and absorbed (EDTA-resistant) fractions and used to calculate differential uptake rates for each metal. The differences in binding and internalisation between different metals indicate different uptake processes exist for each metal. For each metal, competitive uptake experiments using 65Zn showed that after 72 h of exposure Zn2+ uptake was reduced by most metals particularly 0.5 μM Cd2+, while 2 μM Co2+ increased Zn2+ uptake. This study demonstrates that N. punctiforme discriminates between different metals and favourably substitutes their uptake to avoid the toxic effects of particular metals.
Language eng
DOI 10.1007/s10534-012-9556-4
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2012, Springer Science+Business Media
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