Infusion with the antioxidant N-acetylcysteine attenuates early adaptive responses to exercise in human skeletal muscle

Petersen, A. C., McKenna, M. J., Medved, I., Murphy, K. T., Brown, M. J., Della Gatta, P. and Cameron-Smith, D. 2012, Infusion with the antioxidant N-acetylcysteine attenuates early adaptive responses to exercise in human skeletal muscle, Acta physiologica, vol. 204, no. 3, pp. 382-392, doi: 10.1111/j.1748-1716.2011.02344.x.

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Title Infusion with the antioxidant N-acetylcysteine attenuates early adaptive responses to exercise in human skeletal muscle
Author(s) Petersen, A. C.
McKenna, M. J.
Medved, I.
Murphy, K. T.
Brown, M. J.
Della Gatta, P.ORCID iD for Della Gatta, P.
Cameron-Smith, D.
Journal name Acta physiologica
Volume number 204
Issue number 3
Start page 382
End page 392
Total pages 11
Publisher Wiley - Blackwell Publishing
Place of publication Oxford, England
Publication date 2012-03
ISSN 1748-1708
Keyword(s) exercise adaptation
mitogen-activated protein kinase
nuclear transcription factor kappa-B
signalling pathways
Summary Aim:  Production of reactive oxygen species (ROS) in skeletal muscle is markedly increased during exercise and may be essential for exercise adaptation. We, therefore, investigated the effects of infusion with the antioxidant N-acetylcysteine (NAC) on exercise-induced activation of signalling pathways and genes involved in exercise adaptation in human skeletal muscle.

Methods:  Subjects completed two exercise tests, 7 days apart, with saline (control, CON) or NAC infusion before and during exercise. Exercise tests comprised of cycling at 71%inline image2peak for 45 min, and then 92% \dot{{V}}\hbox{O}2peak to fatigue, with vastus lateralis biopsies at pre-infusion, after 45-min cycling and at fatigue.

Results:  Analysis was conducted on the mitogen-activated protein kinase signalling pathways, demonstrating that NAC infusion blocked the exercise-induced increase in JNK phosphorylation, but not ERK1/2, or p38 MAPK. Nuclear factor-κB p65 phosphorylation was unaffected by exercise; however, it was reduced in NAC at fatigue by 14% (P < 0.05) compared with pre-infusion. Analysis of exercise and/or ROS-sensitive genes demonstrated that exercise-induced mRNA expression is ROS dependent of MnSOD, but not PGC-1α, interleukin-6, monocyte chemotactic protein-1, or heat-shock protein 70.

Conclusion:  These results suggest that inhibition of ROS attenuates some skeletal muscle cell signalling pathways and gene expression involved in adaptations to exercise.
Language eng
DOI 10.1111/j.1748-1716.2011.02344.x
Field of Research 110602 Exercise Physiology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, The Authors. Acta Physiologica 2011 Scandinavian Physiological Society
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Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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