Molecular identification of marine yeast and its spectroscopic analysis establishes unsaturated fatty acid accumulation

Gupta, Adarsha, Vongsvivut, Jitraporn, Barrow, Colin J. and Puri, Munish 2012, Molecular identification of marine yeast and its spectroscopic analysis establishes unsaturated fatty acid accumulation, Journal of bioscience and bioengineering, vol. 114, no. 4, pp. 411-417.

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Title Molecular identification of marine yeast and its spectroscopic analysis establishes unsaturated fatty acid accumulation
Author(s) Gupta, Adarsha
Vongsvivut, Jitraporn
Barrow, Colin J.
Puri, Munish
Journal name Journal of bioscience and bioengineering
Volume number 114
Issue number 4
Start page 411
End page 417
Total pages 7
Publisher Elsevier BV
Place of publication Amsterdam, The Netherlands
Publication date 2012-10
ISSN 1347-4421
Keyword(s) marine
oleaginous
unsaturated fatty acids
omega-3
fourier transform infrared spectroscopy (FTIR)
Summary Marine microbes are competent organisms, some of which can accumulate large amounts of lipids. A yeast strain, Rhodotorula mucilaginosa AMCQ8A was isolated from the marine water of the Queenscliff region, Victoria, Australia. The yeast isolate was identified by sequencing 18s rDNA genes. Scanning electron microscopy images revealed scars on the surface of the yeast cells. The Fourier transform infrared spectroscopy microspectroscopy studies demonstrated the presence of unsaturated fatty acids by differential microscopic analysis. The sharp band at 1745 cm-1 was represented by ν(C=O) stretches of ester functional groups from lipids and fats, and therefore indicated the presence of total lipids produced by the cells. Over 65% of the fatty acids from the yeast strain were analyzed as C16 and C18:1 with omega-3 content from about 6% to 7%. Thus, this marine-derived yeast could be a potential source of lipids, including omega-3 fatty acids. 2012, The Society for Biotechnology, Japan. All rights reserved.
Notes Available online 20 June 2012
Language eng
Field of Research 100303 Industrial Biotechnology
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047295

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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Created: Tue, 21 Aug 2012, 17:53:21 EST by Munish Puri

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