Plant extract assisted extracellular tannase production by Aspergillus sp MIK 23

Kaur, Inderdeep and Puri, Munish 2012, Plant extract assisted extracellular tannase production by Aspergillus sp MIK 23, Journal of pure and applied microbiology, vol. 6, no. 1, pp. 185-194.

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Title Plant extract assisted extracellular tannase production by Aspergillus sp MIK 23
Author(s) Kaur, Inderdeep
Puri, Munish
Journal name Journal of pure and applied microbiology
Volume number 6
Issue number 1
Start page 185
End page 194
Total pages 10
Publisher Oriental Scientific Publishing Company
Place of publication Bhopal, India
Publication date 2012-03-14
ISSN 0973-7510
Keyword(s) plant extracts
tannins
optimization
Terminalia chebula
Summary An extracellular tannase (E.C. 3.1.1.20) producing fungal strain was isolated from soil and identified as Aspergillus sp MIK23. Out of various plant extracts, Terminalia chebula powder (TCP) in the optimized medium enhanced enzyme production. Maximum yield of tannase (3 IU ml-1) was obtained with glucose (10 g/L), urea (2 g/L), and yeast extract (2.5 g/L) when inoculated with 10% inoculum in 48 h. An initial medium at pH 6.0 and a cultivation temperature of 37 0C was found to be optimum for enzyme production. Metal ions Mg2+, Zn2+, Ca2+, Cu2+ and Cd2+ did not improve enzyme activity, whereas, Ca2+, Fe2+ and Hg2+ repressed enzyme activity. The enzyme was purified using ammonium sulfate precipitation followed by Q-sepharose ion-exchange chromatography. The enzyme was purified to 42-fold with an overall recovery of 20. The pH and temperature optima of the purified tannase were found to be 7.0 and 37°C, respectively.
Language eng
Field of Research 100304 Industrial Biotechnology Diagnostics (incl Biosensors)
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047297

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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Created: Wed, 22 Aug 2012, 10:09:27 EST by Munish Puri

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