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Differential effect of inbred mouse strain (C57BL/6, DBA/2, 129T2) on insulin secretory function in response to a high fat diet

Andrikopoulos, Sofianos, Massa, Christine M., Aston-Mourney, Kathryn, Funkat, Alexandra, Fam, Barbara C., Hull, Rebecca L., Kahn, Steven E. and Proietto, Joseph 2005, Differential effect of inbred mouse strain (C57BL/6, DBA/2, 129T2) on insulin secretory function in response to a high fat diet, Journal of endocrinology, vol. 187, no. 1, pp. 45-53.

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Title Differential effect of inbred mouse strain (C57BL/6, DBA/2, 129T2) on insulin secretory function in response to a high fat diet
Author(s) Andrikopoulos, Sofianos
Massa, Christine M.
Aston-Mourney, Kathryn
Funkat, Alexandra
Fam, Barbara C.
Hull, Rebecca L.
Kahn, Steven E.
Proietto, Joseph
Journal name Journal of endocrinology
Volume number 187
Issue number 1
Start page 45
End page 53
Total pages 9
Publisher BioScientifica
Place of publication Bristol, England
Publication date 2005-10
ISSN 0022-0795
1479-6805
Keyword(s) animals
arginine
breeding
dietary fats
fasting
glucose
insulin
islets of langerhans
mice
inbred mice
C57BL
species specificity
Summary The increasing production of genetically-modified mouse models has necessitated studies to determine the inherent physiological characteristics of commonly used mouse strains. In this study we examined insulin secretory function in response to an intravenous bolus of glucose or glucose plus arginine in anesthetized C57BL/6, DBA/2 and 129T2 mice fed either a control or high fat diet for 6 weeks. The results show that 129T2 mice had higher fasting plasma glucose levels and lower fasting plasma insulin levels compared with C57BL/6 and DBA/2 mice regardless of diet. Furthermore, 129T2 mice were glucose intolerant and secreted significantly less insulin in response to glucose and glucose plus arginine irrespective of diet compared with the other two strains of mice. DBA/2 mice hypersecreted insulin in response to glucose and glucose plus arginine compared with C57BL/6 and 129T2 mice. Moreover while first phase insulin secretion was appropriately increased in response to the high fat diet in C57BL/6 and 129T2 mice, this was not the case for DBA/2 mice. Mean islet area was decreased in response to a high fat diet in DBA/2 mice, while there was no dietary effect on the other two strains. This study highlights the inherent genetic differences that exist among seemingly normal strains of mice that are commonly used to make transgenic and knockout mice. Understanding these differences will provide researchers with the information to choose the appropriate genetic background on which to express their particular genetic alteration.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005, BioScientifica
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047511

Document type: Journal Article
Collection: School of Medicine
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Created: Thu, 30 Aug 2012, 09:37:48 EST