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DNA found in human immunodeficiency virus type 1 particles may not be required for infectivity

Arts, Eric J., Mak, Johnson, Kleiman, Lawrence and Wainberg, Mark A. 1994, DNA found in human immunodeficiency virus type 1 particles may not be required for infectivity, Journal of general virology, vol. 75, no. 7, pp. 1605-1613.

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Title DNA found in human immunodeficiency virus type 1 particles may not be required for infectivity
Author(s) Arts, Eric J.
Mak, Johnson
Kleiman, Lawrence
Wainberg, Mark A.
Journal name Journal of general virology
Volume number 75
Issue number 7
Start page 1605
End page 1613
Total pages 9
Publisher Society for General Microbiology
Place of publication London, England
Publication date 1994-08
ISSN 0022-1317
1465-2099
Summary We have studied the presence and significance of retroviral genome-derived DNA in the core of human immunodeficiency virus (HIV) particles produced from transfections of HXB2 expression vectors in COS-7 cells and from HIV type 1 IIIB chronically infected H9 cells. Viruses purified by sucrose cushion centrifugation and treated with DNase I contained 1000-fold more viral RNA than DNA. However protease-defective viruses that contained only pl60 ga~p°z had less than 100 times the amount of DNA in their cores than wild-type viruses suggesting that the p66/p51 form of reverse transcriptase was responsible for DNA transcription. Viruses produced by transfections in the presence of 3'-azido-3'-deoxythymidine (AZT) contained the viral RNA genome but only DNA of premature length because of the chain terminating effects of AZT. However such viruses were as infectious for CD4 + cells as wild-type virus. We conclude that retrovirus-derived DNA in HIV-1 particles is not required for infection and does not play a significant role in this process.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©1994, Society for General Microbiology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047512

Document type: Journal Article
Collection: School of Medicine
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