DNA found in human immunodeficiency virus type 1 particles may not be required for infectivity Arts, Eric J., Mak, Johnson, Kleiman, Lawrence and Wainberg, Mark A. 1994, DNA found in human immunodeficiency virus type 1 particles may not be required for infectivity, Journal of general virology, vol. 75, no. 7, pp. 1605-1613, doi: 10.1099/0022-1317-75-7-1605. Attached Files Name Description MIMEType Size Downloads Title DNA found in human immunodeficiency virus type 1 particles may not be required for infectivity Author(s) Arts, Eric J. Mak, Johnson orcid.org/0000-0002-5229-5707 Kleiman, Lawrence Wainberg, Mark A. Journal name Journal of general virology Volume number 75 Issue number 7 Start page 1605 End page 1613 Total pages 9 Publisher Society for General Microbiology Place of publication London, England Publication date 1994-08 ISSN 0022-1317 1465-2099 Summary We have studied the presence and significance of retroviral genome-derived DNA in the core of human immunodeficiency virus (HIV) particles produced from transfections of HXB2 expression vectors in COS-7 cells and from HIV type 1 IIIB chronically infected H9 cells. Viruses purified by sucrose cushion centrifugation and treated with DNase I contained 1000-fold more viral RNA than DNA. However protease-defective viruses that contained only pl60 ga~p°z had less than 100 times the amount of DNA in their cores than wild-type viruses suggesting that the p66/p51 form of reverse transcriptase was responsible for DNA transcription. Viruses produced by transfections in the presence of 3'-azido-3'-deoxythymidine (AZT) contained the viral RNA genome but only DNA of premature length because of the chain terminating effects of AZT. However such viruses were as infectious for CD4 + cells as wild-type virus. We conclude that retrovirus-derived DNA in HIV-1 particles is not required for infection and does not play a significant role in this process. Language eng DOI 10.1099/0022-1317-75-7-1605 Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©1994, Society for General Microbiology Persistent URL http://hdl.handle.net/10536/DRO/DU:30047512 Document type: Journal Article Collection: School of Medicine Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 30 Aug 2012, 09:37:55 EST Thu, 30 Aug 2012, 10:36:24 EST Thu, 13 Sep 2012, 13:58:51 EST Tue, 15 Apr 2014, 14:24:45 EST Fri, 13 Jun 2014, 15:07:13 EST Sat, 17 Jun 2017, 03:12:39 EST Filtered Full Citation counts:   Cited 33 times in TR Web of Science Cited 33 times in Scopus Search Google Scholar Access Statistics: 230 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Thu, 30 Aug 2012, 09:37:55 EST

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