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Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1

Hill, M. K., Hooker, C. W., Harrich, D., Crowe, S. M. and Mak, J. 2001, Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1, Journal of virology, vol. 75, no. 15, pp. 6835-6840, doi: 10.1128/JVI.75.15.6835-6840.2001.

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Title Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1
Author(s) Hill, M. K.
Hooker, C. W.
Harrich, D.
Crowe, S. M.
Mak, J.ORCID iD for Mak, J. orcid.org/0000-0002-5229-5707
Journal name Journal of virology
Volume number 75
Issue number 15
Start page 6835
End page 6840
Total pages 6
Publisher American Society for Microbiology
Place of publication Washington, D. C.
Publication date 2001-08
ISSN 0022-538X
1098-5514
Keyword(s) fusion proteins
gag-pol
gene products
gag
HIV-1
protein biosynthesis
RNA
messenger
viral RNA
genetic transcription
transfection
virus assembly
Summary The intracellular trafficking and subsequent incorporation of Gag-Pol into human immunodeficiency virus type 1 (HIV-1) remains poorly defined. Gag-Pol is encoded by the same mRNA as Gag and is generated by ribosomal frameshifting. The multimerization of Gag and Gag-Pol is an essential step in the formation of infectious viral particles. In this study, we examined whether the interaction between Gag and Gag-Pol is initiated during protein translation in order to facilitate the trafficking and subsequent packaging of Gag-Pol into the virion. A conditional cotransfection system was developed in which virion formation required the coexpression of two HIV-1-based plasmids, one that produces both Gag and Gag-Pol and one that only produces Gag-Pol. The Gag-Pol proteins were either immunotagged with a His epitope or functionally tagged with a mutation (K65R) in reverse transcriptase that is associated with drug resistance. Gag-Pol packaging was assessed to determine whether the Gag-Pol incorporated into the virion was preferentially packaged from the plasmid that expressed both Gag and Gag-Pol or whether it could be packaged from either plasmid. Our data show that translation of Gag and Gag-Pol from the same mRNA is not critical for virion packaging of the Gag-Pol polyprotein or for viral function.
Language eng
DOI 10.1128/JVI.75.15.6835-6840.2001
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2001, American Society for Microbiology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047532

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.