Mature reverse transcriptase (p66/p51) is responsible for low levels of viral DNA found in human immunodeficiency virus type 1 (HIV-1)

Arts, E. J., Mak, J., Kleiman, L. and Wainberg, M. A. 1994, Mature reverse transcriptase (p66/p51) is responsible for low levels of viral DNA found in human immunodeficiency virus type 1 (HIV-1), Leukemia, vol. 8, no. Supplement 1, pp. S175-S178.


Title Mature reverse transcriptase (p66/p51) is responsible for low levels of viral DNA found in human immunodeficiency virus type 1 (HIV-1)
Author(s) Arts, E. J.
Mak, J.
Kleiman, L.
Wainberg, M. A.
Journal name Leukemia
Volume number 8
Issue number Supplement 1
Start page S175
End page S178
Total pages 4
Publisher Nature Publishing Group
Place of publication London, England
Publication date 1994-04
ISSN 0887-6924
1476-5551
Keyword(s) viral DNA analysis
HIV-1 genetics
RNA-directed DNA polymerase physiology
cell line
HIV reverse transcriptase
Humans
viral RNA analysis
Summary Reverse transcription of the HIV RNA genome is thought to occur in the host cell cytoplasm after viral adsorption. However, viral DNA has been isolated in cell-free virus particles. We have quantitated by polymerase chain reaction (PCR) amplification the amount of viral DNA in virions as compared to RNA. Virus produced by proviral DNA transfections of cos-7 cells or by chronically-infected H9 cells; neither of which express the cell surface CD4 receptor, contained at least 1000 times more viral RNA than DNA. In contrast, only 60 times more RNA than DNA was present in virus particles produced by transfection of Jurkat cells, which were CD4-positive and thus potentially susceptible to superinfection. Protease-defective virus, carrying only the precursor of reverse transcriptase (RT) p160gag-pol, contained virtually no detectable DNA. These results indicate that only mature RT (p66/p51) and not its precursor (p160gag-pol) is responsible for the presence of viral DNA in HIV.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047544

Document type: Journal Article
Collection: School of Medicine
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