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Labeling of multiple HIV-1 proteins with the biarsenical-tetracysteine system

Pereira, Candida F., Ellenberg, Paula C., Jones, Kate L., Fernandez, Tara L., Smyth, Redmond P., Hawkes, David J., Hijnen, Marcel, Vivet-Boudou, Valerie, Marquet, Roland, Johnson, Iain and Mak, Johnson 2011, Labeling of multiple HIV-1 proteins with the biarsenical-tetracysteine system, PLoS one, vol. 6, no. 2, pp. 1-10, doi: 10.1371/journal.pone.0017016.

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Title Labeling of multiple HIV-1 proteins with the biarsenical-tetracysteine system
Author(s) Pereira, Candida F.
Ellenberg, Paula C.
Jones, Kate L.
Fernandez, Tara L.
Smyth, Redmond P.
Hawkes, David J.
Hijnen, Marcel
Vivet-Boudou, Valerie
Marquet, Roland
Johnson, Iain
Mak, JohnsonORCID iD for Mak, Johnson orcid.org/0000-0002-5229-5707
Journal name PLoS one
Volume number 6
Issue number 2
Start page 1
End page 10
Total pages 10
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2011
ISSN 1932-6203
Keyword(s) chemical labeling
controlled study
fluorescence
Human immunodeficiency virus 1
nonhuman
T lymphocyte
virus cell interaction
virus infectivity
virus mutant
Summary Due to its small size and versatility, the biarsenical-tetracysteine system is an attractive way to label viral proteins for live cell imaging. This study describes the genetic labeling of the human immunodeficiency virus type 1 (HIV-1) structural proteins (matrix, capsid and nucleocapsid), enzymes (protease, reverse transcriptase, RNAse H and integrase) and envelope glycoprotein 120 with a tetracysteine tag in the context of a full-length virus. We measure the impact of these modifications on the natural virus infection and, most importantly, present the first infectious HIV-1 construct containing a fluorescently-labeled nucleocapsid protein. Furthermore, due to the high background levels normally associated with the labeling of tetracysteine-tagged proteins we have also optimized a metabolic labeling system that produces infectious virus containing the natural envelope glycoproteins and specifically labeled tetracysteine-tagged proteins that can easily be detected after virus infection of T-lymphocytes. This approach can be adapted to other viral systems for the visualization of the interplay between virus and host cell during infection.
Language eng
DOI 10.1371/journal.pone.0017016
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2011, Public Library of Science
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047591

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.