An antiviral response directed by PKR phosphorylation of the RNA helicase A Sadler, Anthony J., Latchoumanin, Olivier, Hawkes, David, Mak, Johnson and Williams, Bryan R. G. 2009, An antiviral response directed by PKR phosphorylation of the RNA helicase A, PLoS pathogens, vol. 5, no. 2, pp. 1-11, doi: 10.1371/journal.ppat.1000311. Attached Files Name Description MIMEType Size Downloads mak-antiviralresponse-2009.pdf Published version application/pdf 849.71KB 152 Title An antiviral response directed by PKR phosphorylation of the RNA helicase A Author(s) Sadler, Anthony J. Latchoumanin, Olivier Hawkes, David Mak, Johnson orcid.org/0000-0002-5229-5707 Williams, Bryan R. G. Journal name PLoS pathogens Volume number 5 Issue number 2 Start page 1 End page 11 Total pages 11 Publisher Public Library of Science Place of publication San Francisco, Calif. Publication date 2009-02 ISSN 1553-7366 1553-7374 Keyword(s) RNA antiviral response PKR phosphorylation immune response Summary The double-stranded RNA-activated protein kinase R (PKR) is a key regulator of the innate immune response. Activation of PKR during viral infection culminates in phosphorylation of the α subunit of the eukaryotic translation initiation factor 2 (eIF2α) to inhibit protein translation. A broad range of regulatory functions has also been attributed to PKR. However, as few additional PKR substrates have been identified, the mechanisms remain unclear. Here, PKR is shown to interact with an essential RNA helicase, RHA. Moreover, RHA is identified as a substrate for PKR, with phosphorylation perturbing the association of the helicase with double-stranded RNA (dsRNA). Through this mechanism, PKR can modulate transcription, as revealed by its ability to prevent the capacity of RHA to catalyze transactivating response (TAR)–mediated type 1 human immunodeficiency virus (HIV-1) gene regulation. Consequently, HIV-1 virions packaged in cells also expressing the decoy RHA peptides subsequently had enhanced infectivity. The data demonstrate interplay between key components of dsRNA metabolism, both connecting RHA to an important component of innate immunity and delineating an unanticipated role for PKR in RNA metabolism. Language eng DOI 10.1371/journal.ppat.1000311 Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2009, Public Library of Science Free to Read? Yes Use Rights Persistent URL http://hdl.handle.net/10536/DRO/DU:30047595 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 30 Aug 2012, 09:47:08 EST Thu, 30 Aug 2012, 10:22:55 EST Tue, 04 Sep 2012, 12:37:02 EST Tue, 04 Sep 2012, 12:40:55 EST Thu, 13 Sep 2012, 09:10:16 EST Fri, 31 Jan 2014, 08:20:03 EST Fri, 13 Jun 2014, 15:12:07 EST Thu, 11 Dec 2014, 18:03:37 EST Fri, 26 Aug 2016, 14:57:30 EST Mon, 05 Sep 2016, 15:42:40 EST Fri, 07 Sep 2018, 12:00:23 EST Filtered Full Citation counts:   Cited 41 times in TR Web of Science Cited 39 times in Scopus Search Google Scholar Access Statistics: 425 Abstract Views, 153 File Downloads  -  Detailed Statistics Created: Thu, 30 Aug 2012, 09:47:08 EST

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