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Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372

Verma, M. L., Chauhan, G. S. and Kanwar, S. S. 2008, Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372, Acta microbiologica et immunologica hungarica, vol. 55, no. 3, pp. 327-342.

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Title Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372
Formatted title Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372
Author(s) Verma, M. L.
Chauhan, G. S.
Kanwar, S. S.
Journal name Acta microbiologica et immunologica hungarica
Volume number 55
Issue number 3
Start page 327
End page 342
Total pages 16
Publisher Akadémiai Kiadó
Place of publication Budapest, Hungary
Publication date 2008-09
ISSN 1217-8950
1588-2640
Keyword(s) bacillus cereus MTCC 8372
immobilization
isopropyl myristate synthesis
lipase
organic solvent and gas liquid chromatography analysis
poly (MAc-co-DMA-cl-MBAm) hydrogel
Summary A purified alkaline thermo-tolerant bacterial lipase from Bacillus cereus MTCC 8372 was immobilized on a Poly (MAc- co -DMA- cl -MBAm) hydrogel. The hydrogel showed approximately 94% binding capacity for lipase. The immobilized lipase (2.36 IU) was used to achieve esterification of myristic acid and isopropanol in n -heptane at 65 °C under continuous shaking. The myristic acid and isopropanol when used at a concentration of 100 mM each in n -heptane resulted in formation of isopropyl myristate (66.0 ± 0.3 mM) in 15 h. The reaction temperature below or higher than 65°C markedly reduced the formation of isopropyl myristate. Addition of a molecular sieve (3 Å × 1.5 mm) to the reaction mixture drastically reduced the ester formation. The hydrogel bound lipase when repetitively used to perform esterification under optimized conditions resulted in 38.0 ± 0.2 mM isopropyl myristate after the 3 rd cycle of esterification.
Language eng
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 860106 Oils and Fats (incl. Margarines)
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2008, Akadémiai Kiadó
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047716

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.