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Purification and characterization of a low molecular mass alkaliphilic lipase of Bacillus cereus MTCC 8372

Verma, M. L. and Kanwar, S. S. 2010, Purification and characterization of a low molecular mass alkaliphilic lipase of Bacillus cereus MTCC 8372, Acta microbiologica et immunologica hungarica, vol. 57, no. 3, pp. 191-207.

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Title Purification and characterization of a low molecular mass alkaliphilic lipase of Bacillus cereus MTCC 8372
Formatted title Purification and characterization of a low molecular mass alkaliphilic lipase of Bacillus cereus MTCC 8372
Author(s) Verma, M. L.
Kanwar, S. S.
Journal name Acta microbiologica et immunologica hungarica
Volume number 57
Issue number 3
Start page 191
End page 207
Total pages 17
Publisher Akadémiai Kiadó
Place of publication Budapest, Hungary
Publication date 2010-09
ISSN 1217-8950
1588-2640
Keyword(s) Bacillus cereus MTCC 8372
biochemical characterization
detergents and PMSF
effect of metal-ions
low molecular mass lipase
purification
Summary A low molecular mass alkaliphilic extra-cellular lipase of Bacillus cereus MTCC 8372 was purified 35-fold by hydrophobic interaction (Octyl-Sepharose) chromatography. The purified enzyme was found to be electrophoretically pure by denaturing gel electrophoresis and possessed a molecular mass of approximately 8 kDa. It is a homopentamer of 40 kDa as revealed by native-PAGE. The lipase was optimally active at 55 °C and retained approximately half of its original activity after 40 min incubation at 55 °C. The enzyme was maximally active at pH 8.5. Mg 2+ , Cu 2+ , Ca 2+ , Hg 2+ , Al 3+ and Fe 3+ at 1 mM enhanced hydrolytic activity of the lipase. Interestingly, Hg 2+ ions synergized and Zn 2+ and Co 2+ ions antagonized the lipase activity. Among surfactants, Tween 80 promoted the lipase activity. Phenyl methyl sulfonyl fluoride (PMSF, 15 mM) decreased 98% of original activity of lipase. The lipase was highly specific towards p -nitrophenyl palmitate and showed a V max and K m of 0.70 mmol.mg −1 .min −1 and 32 mM for hydrolysis of p NPP.

Language eng
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 860106 Oils and Fats (incl. Margarines)
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2010, Akadémiai Kiadó
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047723

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.