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Development of twenty-four novel microsatellite markers for the freshwater crayfish, Geocharax gracilis, using next generation sequencing

Sherman, Craig, D. H., Ierodiaconou, Daniel, Stanley, Annalise M., Weston, Kim, Gardner, Michael G. and Schultz, Mark B. 2012, Development of twenty-four novel microsatellite markers for the freshwater crayfish, Geocharax gracilis, using next generation sequencing, Conservation genetics resources, vol. 4, no. 3, pp. 555-558.

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Title Development of twenty-four novel microsatellite markers for the freshwater crayfish, Geocharax gracilis, using next generation sequencing
Formatted title  Development of twenty-four novel microsatellite markers for the freshwater crayfish, Geocharax gracilis, using next generation sequencing
Author(s) Sherman, Craig, D. H.
Ierodiaconou, Daniel
Stanley, Annalise M.
Weston, Kim
Gardner, Michael G.
Schultz, Mark B.
Journal name Conservation genetics resources
Volume number 4
Issue number 3
Start page 555
End page 558
Total pages 4
Publisher Springer, Netherlands
Place of publication Dordrecht, The Netherlands
Publication date 2012
ISSN 1877-7252
1877-7260
Keyword(s) Crustacean
Dispersal
Genetic structure
Mating system
Gene flow
Summary The crayfish Geocharax gracilis is an important inhabitant of natural and agricultural drainage systems in south-eastern Australia. To investigate population structure, genetic diversity and patterns of connectivity in natural and human-altered ecosystems, we isolated and characterised 24 microsatellite loci using next generation sequencing. Loci were initially tested for levels of variation based on 12 individuals from across the species’ geographical range. A further 33 individuals from a single population were used to test for departures from Hardy–Weinberg equilibrium and linkage disequilibrium. We detected high to moderate levels of genetic variation across most loci with a mean allelic richness of 8.42 and observed heterozygosity of 0.629 (all samples combined). We found no evidence for linkage disequilibrium between any loci and only three loci (Geo01, Geo24 and Geo47) showed significant deviations from Hardy–Weinberg expectations. These same three loci, plus two additional loci (Geo06 and Geo28), also showed the presence of null alleles. These 24 variable markers will provide an important tool for future population genetic assessments in natural and human altered environments.
Language eng
Field of Research 060411 Population, Ecological and Evolutionary Genetics
060207 Population Ecology
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047791

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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