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Enzyme-assisted self-assembly under thermodynamic control

Williams, Richard J., Smith, Andrew M., Collins, Richard, Hodson, Nigel, Das, Apurba K. and Ulijn, Rein V. 2009, Enzyme-assisted self-assembly under thermodynamic control, Nature nanotechnology, vol. 4, no. 1, pp. 19-24, doi: 10.1038/nnano.2008.378.

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Title Enzyme-assisted self-assembly under thermodynamic control
Author(s) Williams, Richard J.
Smith, Andrew M.
Collins, Richard
Hodson, Nigel
Das, Apurba K.
Ulijn, Rein V.
Journal name Nature nanotechnology
Volume number 4
Issue number 1
Start page 19
End page 24
Total pages 6
Publisher Nature Publishing Group
Place of publication London, England
Publication date 2009-01
ISSN 1748-3387
Keyword(s) amino acids
secondary protein structure
Summary The production of functional molecular architectures through self-assembly is commonplace in biology, but despite advances1, 2, 3, it is still a major challenge to achieve similar complexity in the laboratory. Self-assembled structures that are reproducible and virtually defect free are of interest for applications in three-dimensional cell culture4, 5, templating6, biosensing7 and supramolecular electronics8. Here, we report the use of reversible enzyme-catalysed reactions to drive self-assembly. In this approach, the self-assembly of aromatic short peptide derivatives9, 10 provides a driving force that enables a protease enzyme to produce building blocks in a reversible and spatially confined manner. We demonstrate that this system combines three features: (i) self-correction—fully reversible self-assembly under thermodynamic control; (ii) component-selection—the ability to amplify the most stable molecular self-assembly structures in dynamic combinatorial libraries11, 12, 13; and (iii) spatiotemporal confinement of nucleation and structure growth. Enzyme-assisted self-assembly therefore provides control in bottom-up fabrication of nanomaterials that could ultimately lead to functional nanostructures with enhanced complexities and fewer defects.
Language eng
DOI 10.1038/nnano.2008.378
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, Macmillan Publishers Limited. All rights reserved.
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Document type: Journal Article
Collection: School of Life and Environmental Sciences
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