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Copy number variation and transposable elements feature in recent, ongoing adaptation at the Cyp6g1 locus

Schmidt, Joshua M., Good, Robert T., Appleton, Belinda, Sherrard, Jayne, Raymant, Greta C., Bogwitz, Michael R., Martin, Jon, Daborn, Phillip J., Goddard, Mike E., Batterham, Philip and Robin, Charles 2010, Copy number variation and transposable elements feature in recent, ongoing adaptation at the Cyp6g1 locus, PLoS genetics, vol. 6, no. 6, pp. 1-11, doi: 10.1371/journal.pgen.1000998.

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Title Copy number variation and transposable elements feature in recent, ongoing adaptation at the Cyp6g1 locus
Author(s) Schmidt, Joshua M.
Good, Robert T.
Appleton, Belinda
Sherrard, Jayne
Raymant, Greta C.
Bogwitz, Michael R.
Martin, Jon
Daborn, Phillip J.
Goddard, Mike E.
Batterham, Philip
Robin, Charles
Journal name PLoS genetics
Volume number 6
Issue number 6
Start page 1
End page 11
Total pages 11
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2010-06
ISSN 1553-7390
Keyword(s) adaptation
controlled study
copy number variation
Drosophila melanogaster
gene frequency
gene locus
genetic variability
geographical variation (species)
insecticide resistance
natural population
phenotypic variation
strain difference
Summary The increased transcription of the Cyp6g1 gene of Drosophila melanogaster, and consequent resistance to insecticides such as DDT, is a widely cited example of adaptation mediated by cis-regulatory change. A fragment of an Accord transposable element inserted upstream of the Cyp6g1 gene is causally associated with resistance and has spread to high frequencies in populations around the world since the 1940s. Here we report the existence of a natural allelic series at this locus of D. melanogaster, involving copy number variation of Cyp6g1, and two additional transposable element insertions (a P and an HMS-Beagle). We provide evidence that this genetic variation underpins phenotypic variation, as the more derived the allele, the greater the level of DDT resistance. Tracking the spatial and temporal patterns of allele frequency changes indicates that the multiple steps of the allelic series are adaptive. Further, a DDT association study shows that the most resistant allele, Cyp6g1-[BP], is greatly enriched in the top 5% of the phenotypic distribution and accounts for ~16% of the underlying phenotypic variation in resistance to DDT. In contrast, copy number variation for another candidate resistance gene, Cyp12d1, is not associated with resistance. Thus the Cyp6g1 locus is a major contributor to DDT resistance in field populations, and evolution at this locus features multiple adaptive steps occurring in rapid succession.
Language eng
DOI 10.1371/journal.pgen.1000998
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2010, Schmidt et al.
Free to Read? Yes
Use Rights Creative Commons Attribution licence
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Document type: Journal Article
Collections: Institute for Frontier Materials
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