O-acetylation of sialic acids in N-glycans of Atlantic salmon (Salmo salar) serum is altered by handling stress

Liu, Xin, Afonso, Luis, Altman, Eleonora, Johnson, Stewart, Brown, Laura and Li, Jianjun 2008, O-acetylation of sialic acids in N-glycans of Atlantic salmon (Salmo salar) serum is altered by handling stress, Proteomics, vol. 8, no. 14, pp. 2849-2857, doi: 10.1002/pmic.200701093.

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Title O-acetylation of sialic acids in N-glycans of Atlantic salmon (Salmo salar) serum is altered by handling stress
Author(s) Liu, Xin
Afonso, LuisORCID iD for Afonso, Luis orcid.org/0000-0002-5905-1131
Altman, Eleonora
Johnson, Stewart
Brown, Laura
Li, Jianjun
Journal name Proteomics
Volume number 8
Issue number 14
Start page 2849
End page 2857
Total pages 9
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Place of publication Weinheim, Germany
Publication date 2008-07
ISSN 1615-9853
1615-9861
Keyword(s) Atlantic salmon
glycomics
glycosylation
O-acetylation
sialic acid
Summary O-acetylation is one of the major modifications of sialic acids that significantly alters biological properties of the parent molecule. These O-acetylated forms are components of the cellular membrane and can affect physiological and pathological responses. Understanding the role of N-glycans in physiology is of increasing relevance to cellular biologists in various disciplines who study glycoproteomics yet lack information regarding the function of the attached glycans. It is well known that stress may decrease immune function in fish; however, there are only few suitable biomarkers available to monitor the physiological responses under the stress conditions. This study is the first report on the effect of stress on the profile of O-acetylation of sialic acids in fish serum. In order to preserve the relevant structural characteristics as much as possible, native N-glycans were directly analyzed using CE-MS. We have characterized the N-glycans in serum of salmon (Salmo salar) exposed to long-term handling stress (15 s out of the water, daily for 4 wk) and compared with the results obtained from sera of control fish. The results indicated that major N-glycans in salmon serum contained mono-acetylated sialic acids (83%), and that the O-acetylation pattern of sialic acids could be altered by long-term stress.
Language eng
DOI 10.1002/pmic.200701093
Field of Research 060804 Animal Immunology
070401 Aquaculture
070405 Fish Physiology and Genetics
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2008, Wiley-Blackwell
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047910

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