Synthesis of ethyl oleate employing synthetic hydrogel-immobilized lipase of Bacillus coagulans MTCC-6375

Kanwar, S. S., Kaushal, R. K., Verma, M. L., Kumar, Y., Azmi, W., Gupta, R., Chimni, S. S. and Chauhan, G. S. 2007, Synthesis of ethyl oleate employing synthetic hydrogel-immobilized lipase of Bacillus coagulans MTCC-6375, Indian journal of biotechnology, vol. 6, no. 1, pp. 68-73.

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Title Synthesis of ethyl oleate employing synthetic hydrogel-immobilized lipase of Bacillus coagulans MTCC-6375
Author(s) Kanwar, S. S.
Kaushal, R. K.
Verma, M. L.
Kumar, Y.
Azmi, W.
Gupta, R.
Chimni, S. S.
Chauhan, G. S.
Journal name Indian journal of biotechnology
Volume number 6
Issue number 1
Start page 68
End page 73
Total pages 6
Publisher National Institute of Science Communication and Information Resources
Place of publication New Delhi, India
Publication date 2007-01
ISSN 0972-5849
0975-0967
Keyword(s) bacillus coagulans MTCC-6375
ethyl oleate synthesis
poly (MAc-co-DMA-cl-MBAm)-immobilized lipase
synthetic hydrogel lipase
Summary Ten polymeric hydrogels were chemically synthesized by varying the concentrations of copolymer (DMA) and cross-linker (MBAm) molecules. An alkaline lipase of Bacillus coagulans MTCC-6375 was immobilized onto a poly (MAc-co-DMA-cl-MBAm)-hydrogel support at pH 8.5 and temperature 55ºC in 16 h. The bound lipase possessed 7.6 U.g⁻¹ (matrix) lipase activity with a specific activity of 18 U.mg⁻¹ protein. Hydrogel bound-lipase catalyzed esterification of oleic acid and ethanol to synthesize ethyl oleate in n-nonane. Various kinetic parameters were optimized to produce ethyl oleate using immobilized lipase. The optimal parameters were bound enzyme/substrate (E/S) ratio 0.62 mg/mM, ethanol/oleic acid 100 mM:75 mM or 100 mM:100 mM, incubation time 18 h and reaction temperature 55ºC that resulted in approximately 53% conversion of reactants into ethyl oleate in n-nonane. However, addition of a molecular sieve to the reaction mixture promoted the conversion to 58% in 18 h in n-nonane, which was equivalent to 55 mM of ethyl oleate produced.
Language eng
Field of Research 100301 Biocatalysis and Enzyme Technology
100306 Industrial Molecular Engineering of Nucleic Acids and Proteins
100703 Nanobiotechnology
Socio Economic Objective 860106 Oils and Fats (incl. Margarines)
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047961

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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