Synthesis of ethyl laurate by hydrogel immobilized lipase of Bacillus coagulans MTCC-6375

Kanwar, S. S., Kaushal, R. K., Verma, M. L., Kumar, Y., Chauhan, G. S., Gupta, R. and Chimni, S. S. 2005, Synthesis of ethyl laurate by hydrogel immobilized lipase of Bacillus coagulans MTCC-6375, Indian journal of microbiology, vol. 45, no. 3, pp. 187-193.

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Title Synthesis of ethyl laurate by hydrogel immobilized lipase of Bacillus coagulans MTCC-6375
Author(s) Kanwar, S. S.
Kaushal, R. K.
Verma, M. L.
Kumar, Y.
Chauhan, G. S.
Gupta, R.
Chimni, S. S.
Journal name Indian journal of microbiology
Volume number 45
Issue number 3
Start page 187
End page 193
Total pages 7
Publisher Springer (India) Private
Place of publication New Delhi, India
Publication date 2005
ISSN 0046-8991
0973-7715
Keyword(s) Bacillus coagulans
Ethyl laurate synthesis
Lipase esterification
Poly (MAc-co-DMA-cl-MBAm)-hydrogel
Summary An alkaline thermo-tolerant lipase from Bacillus coagulans MTCC-6375 was purified and efficiently immobilized onto a synthetic hydrophobic poly (MAc-co-DMA-cl-MBAm)-hydrogel at pH 8.5 and temperature 55°C in 16 h. The hydrogel bound matrix possessed 7.6 IU g -1 matrix lipase activity with a specific activity of 18 IU mg -1 protein. Immobilized lipase was used to catalyze the esterification of lauric acid and ethanol to produce ethyl laurate in n-nonane. The reaction conditions that were optimized to produce ethyl laurate in n-nonane included enzyme/substrate (E/S) ratio, substrate concentration, reaction time and reaction temperature. The optimized parameters were E/S ratio of 0.5 mg mM -1, ethanol:lauric acid in ratio of 100 mM:100 mM and reaction time of 15 h at 65°C under continuous shaking (200 rpm). Optimized conditions resulted in 66% conversion of reactants into ethyl laurate in n-nonane in the presence of 300 mg molecular sieve mL -1 reaction mixture.
Language eng
Field of Research 100301 Biocatalysis and Enzyme Technology
100306 Industrial Molecular Engineering of Nucleic Acids and Proteins
100703 Nanobiotechnology
Socio Economic Objective 860106 Oils and Fats (incl. Margarines)
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30047962

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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