Retention mechanism divergence of a mixed mode stationary phase for high performance liquid chromatography

Stevenson, Paul G., Fairchild, Jacob N. and Guiochon, Georges 2011, Retention mechanism divergence of a mixed mode stationary phase for high performance liquid chromatography, Journal of chromatography A, vol. 1218, no. 14, pp. 1822-1827, doi: 10.1016/j.chroma.2011.01.078.

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Title Retention mechanism divergence of a mixed mode stationary phase for high performance liquid chromatography
Author(s) Stevenson, Paul G.ORCID iD for Stevenson, Paul G.
Fairchild, Jacob N.
Guiochon, Georges
Journal name Journal of chromatography A
Volume number 1218
Issue number 14
Start page 1822
End page 1827
Total pages 6
Publisher Elsevier BV
Place of publication Amsterdam, The Netherlands
Publication date 2011-04-08
ISSN 0021-9673
Keyword(s) β-Lactoglobulin digest
mixed mode stationary phase
mobile phase pH
Scherzo SM-C18
Summary Mixed mode stationary phases utilize secondary retention mechanisms to add a dimensionality to the surface of high performance liquid chromatography (HPLC) adsorbents. This approach was used by several authors to improve the separation performance of single dimension separations. We explored the magnitude of these secondary interactions by performing an off-line two-dimensional (2D)-HPLC separation with a Scherzo SM-C18 column of a β-lactoglobulin tryptic digest with a mobile phase pH of 7 in the first dimension and 2 in the second. Mechanism divergence was determined using the peak capacity and a geometric approach to factor analysis, to measure the correlation. This separation was repeated with a C18 stationary phase as a control. It was found that the C18 column had a correlation coefficient of 0.784, smaller than the mixed mode column, 0.884. This indicated that the retention mechanisms of the C18 column were more divergent under these two pH environments than the mixed mode column. However, the SM-C18 still provided alternative selectivity of the peptides to that of the C18 and could be considered as a good alternative for further 2D-HPLC separations.
Language eng
DOI 10.1016/j.chroma.2011.01.078
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2011, Elsevier B.V.
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Document type: Journal Article
Collection: School of Life and Environmental Sciences
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