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Oxygen consumption and blood flow distribution in perfused skeletal muscle of chinook salmon

Forgan, Leonard G. and Forster, Malcolm E. 2009, Oxygen consumption and blood flow distribution in perfused skeletal muscle of chinook salmon, Journal of comparative physiology B : biochemical, systemic, and environmental physiology, vol. 179, no. 3, pp. 359-368, doi: 10.1007/s00360-008-0320-6.

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Title Oxygen consumption and blood flow distribution in perfused skeletal muscle of chinook salmon
Author(s) Forgan, Leonard G.
Forster, Malcolm E.
Journal name Journal of comparative physiology B : biochemical, systemic, and environmental physiology
Volume number 179
Issue number 3
Start page 359
End page 368
Total pages 10
Publisher Springer
Place of publication Berlin, Germany
Publication date 2009
ISSN 0174-1578
1432-136X
Keyword(s) blood flow
oxygen consumption
perfusion
salmon
skeletal muscle
Summary An isolated, perfused salmon tail preparation showed oxyconformance at low oxygen delivery rates. Addition of pig red blood cells to the perfusing solution at a haematocrit of 5 or 10% allowed the tail tissues to oxyregulate. Below ca. 60 ml O2 kg−1 h−1 of oxygen delivery (DO2), VO2 was delivery dependent. Above this value additional oxygen delivery did not increase VO2 of resting muscle above ca. 35 ml O2 kg−1 h−1. Following electrical stimulation, VO2 increased to ca. 65 ml O2 kg−1 h−1, with a critical DO2 of ca. 150 ml O2 kg−1 h−1. Dorsal aortic pressure fell to 69% of the pre-stimulation value after 5 min of stimulation and to 54% after 10 min. Microspheres were used to determine blood flow distribution (BFD) to red (RM) and white muscle (WM) within the perfused myotome. Mass specific BFD ratio at rest was found to be 4.03 ± 0.49 (RM:WM). After 5 min of electrical stimulation the ratio did not change. Perfusion with saline containing the tetrazolium salt 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) revealed significantly more mitochondrial activity in RM. Formazan production from MTT was directly proportional to time of perfusion in both red and WM. The mitochondrial activity ratio (RM:WM) did not change over 90 min of perfusion.
Language eng
DOI 10.1007/s00360-008-0320-6
Field of Research 060604 Comparative Physiology
060603 Animal Physiology - Systems
060809 Vertebrate Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2008, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048001

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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