Effects of π-π interactions on the separation of PAHs on phenyl-type stationary phases

Stevenson, Paul G., Kayillo, Sindy, Dennis, Gary R. and Shalliker, R. Andrew 2008, Effects of π-π interactions on the separation of PAHs on phenyl-type stationary phases, Journal of liquid chromatography and related technologies, vol. 31, no. 3, pp. 324-347.

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Title Effects of π-π interactions on the separation of PAHs on phenyl-type stationary phases
Author(s) Stevenson, Paul G.
Kayillo, Sindy
Dennis, Gary R.
Shalliker, R. Andrew
Journal name Journal of liquid chromatography and related technologies
Volume number 31
Issue number 3
Start page 324
End page 347
Total pages 24
Publisher Taylor and Francis
Place of publication Philadelphia, Pa.
Publication date 2008-01
ISSN 1082-6076
1520-572X
Keyword(s) π-π interactions
cosmosil 5PBB
PAH
retention characteristics
stationary phase
synergi polar-RP
Summary Phenyl‐type stationary phase surfaces are useful for the separation of highly aromatic compounds because of the extensive intermolecular forces between the π‐electron systems. For this reason, we studied the retention behaviour and selectivity of polycyclic aromatic hydrocarbons (PAHs) on Synergi polar‐RP and Cosmosil 5PBB chromatography columns using methanol/water, acetonitrile/water, benzene spiked (0.5%) methanol/water, and benzene spiked (0.5%) acetonitrile/water mobile phases. These four solvent systems were employed because π‐π. interactions between the aromatic solute (i.e., PAH) and the aromatic stationary phase should be inhibited in mobile phases that are also π electron rich, and hence a competitor for the analyte. Our results showed that the acetonitrile mobile phases were substantially stronger eluents than the methanol mobile phases, which was consistent with the premise that retention of aromatic compounds is sensitive to π‐π. interactions. Aside from changes in absolute retention, selectivity of the PAHs was also generally greater in methanol rather than acetonitrile mobile phases because the methanol did not attenuate the π‐π. bonding interactions between the PAH and the stationary phase; but, despite this, the retention behaviour of the Synergi polar‐RP column was similar to that observed on C18 columns. The excessive retention times of the Cosmosil 5PBB column were decreased dramatically when acetonitrile was used as the mobile phase; however, selectivity between structural isomers was lost.
Language eng
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2008, Taylor and Francis Group, LLC
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048027

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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