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Force amplification response of actin filaments under confined compression

Greene, George W., Anderson, Travers H., Zeng, Hongbo, Zappone, Bruno and Israelachvili, Jacob N. 2009, Force amplification response of actin filaments under confined compression, National academy of sciences. Proceedings, vol. 106, no. 2, pp. 445-449.

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Title Force amplification response of actin filaments under confined compression
Author(s) Greene, George W.ORCID iD for Greene, George W. orcid.org/0000-0003-2250-8334
Anderson, Travers H.
Zeng, Hongbo
Zappone, Bruno
Israelachvili, Jacob N.
Journal name National academy of sciences. Proceedings
Volume number 106
Issue number 2
Start page 445
End page 449
Total pages 5
Publisher National Academy of Sciences
Place of publication Washington, D. C.
Publication date 2009-01-13
ISSN 0027-8424
1091-6490
Keyword(s) elasticity
fluctuation and dynamic
mechanotransduction
stiffening
Summary Actin protein is a major component of the cell cytoskeleton, and its ability to respond to external forces and generate propulsive forces through the polymerization of filaments is central to many cellular processes. The mechanisms governing actin's abilities are still not fully understood because of the difficulty in observing these processes at a molecular level. Here, we describe a technique for studying actin–surface interactions by using a surface forces apparatus that is able to directly visualize and quantify the collective forces generated when layers of noninterconnected, end-tethered actin filaments are confined between 2 (mica) surfaces. We also identify a force-response mechanism in which filaments not only stiffen under compression, which increases the bending modulus, but more importantly generates opposing forces that are larger than the compressive force. This elastic stiffening mechanism appears to require the presence of confining surfaces, enabling actin filaments to both sense and respond to compressive forces without additional mediating proteins, providing insight into the potential role compressive forces play in many actin and other motor protein-based phenomena.
Language eng
Field of Research 029901 Biological Physics
030603 Colloid and Surface Chemistry
090301 Biomaterials
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048055

Document type: Journal Article
Collection: Institute for Frontier Materials
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