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Adsorption, lubrication, and wear of lubricin on model surfaces : polymer brush-like behavior of a glycoprotein

Zappone, Bruno, Ruths, Marina, Greene, George W., Jay, Gregory D. and Israelachvili, Jacob N. 2007, Adsorption, lubrication, and wear of lubricin on model surfaces : polymer brush-like behavior of a glycoprotein, Biophysical journal, vol. 92, no. 5, pp. 1693-1708.

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Title Adsorption, lubrication, and wear of lubricin on model surfaces : polymer brush-like behavior of a glycoprotein
Author(s) Zappone, Bruno
Ruths, Marina
Greene, George W.ORCID iD for Greene, George W. orcid.org/0000-0003-2250-8334
Jay, Gregory D.
Israelachvili, Jacob N.
Journal name Biophysical journal
Volume number 92
Issue number 5
Start page 1693
End page 1708
Total pages 16
Publisher Cell Press
Place of publication St. Louis, Mo.
Publication date 2007-03-01
ISSN 0006-3495
1542-0086
Keyword(s) aluminum silicates
glycoproteins
humans
hydrophobicity
lubrication
polylysine
polymers
shear strength
surface properties
synovial fluid
Summary Using a surface force apparatus, we have measured the normal and friction forces between layers of the human glycoprotein lubricin, the major boundary lubricant in articular joints, adsorbed from buffered saline solution on various hydrophilic and hydrophobic surfaces: i), negatively charged mica, ii), positively charged poly-lysine and aminothiol, and iii), hydrophobic alkanethiol monolayers. On all these surfaces lubricin forms dense adsorbed layers of thickness 60–100 nm. The normal force between two surfaces is always repulsive and resembles the steric entropic force measured between layers of end-grafted polymer brushes. This is the microscopic mechanism behind the antiadhesive properties showed by lubricin in clinical tests. For pressures up to ∼6 atm, lubricin lubricates hydrophilic surfaces, in particular negatively charged mica (friction coefficient μ = 0.02–0.04), much better than hydrophobic surfaces (μ > 0.3). At higher pressures, the friction coefficient is higher (μ > 0.2) for all surfaces considered and the lubricin layers rearrange under shear. However, the glycoprotein still protects the underlying substrate from damage up to much higher pressures. These results support recent suggestions that boundary lubrication and wear protection in articular joints are due to the presence of a biological polyelectrolyte on the cartilage surfaces.
Language eng
Field of Research 029901 Biological Physics
030399 Macromolecular and Materials Chemistry not elsewhere classified
090301 Biomaterials
Socio Economic Objective 920116 Skeletal System and Disorders (incl. Arthritis)
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, by the Biophysical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048058

Document type: Journal Article
Collection: Institute for Frontier Materials
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