Internal morphological analysis for age estimation of blow fly pupae (Diptera : calliphoridae) in postmortem interval estimation

Davies, Katherine and Harvey, Michelle L. 2013, Internal morphological analysis for age estimation of blow fly pupae (Diptera : calliphoridae) in postmortem interval estimation, Journal of forensic sciences, vol. 58, no. 1, pp. 79-84.

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Title Internal morphological analysis for age estimation of blow fly pupae (Diptera : calliphoridae) in postmortem interval estimation
Author(s) Davies, Katherine
Harvey, Michelle L.
Journal name Journal of forensic sciences
Volume number 58
Issue number 1
Start page 79
End page 84
Total pages 6
Publisher Wiley - Blackwell Publishing
Place of publication Hoboken, N. J.
Publication date 2013
ISSN 0022-1198
1556-4029
Keyword(s) forensic science
forensic entomology
Calliphoridae
pupae histology
postmortem interval
Summary Larvae and pupae of blow fly species are frequently used in postmortem interval estimation, their age indicating minimum time since death. Most studies have considered age estimation of larvae, neglecting study of pupae. Relative development of external pupal features is useful, but there are also internal changes during metamorphosis that may be indicators of age, utilizing histological techniques. This study aimed to optimize preservation and histological analysis of blow fly pupae, specifically Calliphora vicina Robineau-Desvoidy and Lucilia sericata (Meigen), and to examine internal features with potential for age estimation. Effect of hot-water-killing and different preservatives were examined. It was determined that blow fly pupae should be pierced through the three tagma, hot-water-killed, and preserved in 80% ethanol as the optimal preservation for subsequent analyses. Hematoxylin and eosin stained pupal sections revealed differences in brain and thoracic muscle development throughout the pupal stage with potential for age estimation.
Language eng
Field of Research 069901 Forensic Biology
Socio Economic Objective 940403 Criminal Justice
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2012, American Academy of Forensic Sciences
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048154

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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