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Effect of sodium selenite-enriched reperfusion solutions on rat cardiac ischemia reperfusion injury

Lymbury, Robyn, Venardos, Kylie and Perkins, Anthony V. 2006, Effect of sodium selenite-enriched reperfusion solutions on rat cardiac ischemia reperfusion injury, Biological trace element research, vol. 114, no. 1-3, pp. 197-206.

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Title Effect of sodium selenite-enriched reperfusion solutions on rat cardiac ischemia reperfusion injury
Author(s) Lymbury, Robyn
Venardos, Kylie
Perkins, Anthony V.
Journal name Biological trace element research
Volume number 114
Issue number 1-3
Start page 197
End page 206
Total pages 10
Publisher Humana Press
Place of publication Totowa, N. J.
Publication date 2006
ISSN 0163-4984
1559-0720
Keyword(s) antioxidants
cardiac ischemia reperfusion
cardioplegia
oxidative stress
selenium
Summary Cardiac surgery often generates oxidative stress leading to ischemia reperfusion injury (I-R). Antioxidants have been shown to prevent this injury and have been added to cardioplegic solutions to assist in recovery. In this study, we tested the effectiveness of sodium selenite in protecting against ischemia reperfusion injury and investigated the mechanisms behind this protection. Hearts from male Wistar rats were subjected to ischemia reperfusion using the Langendorf model. Krebs-Henseleit perfusion solutions were supplemented with 0,0.1, 0.5, 1.0, and 10μM sodium selenite. Hearts were perfused for 30 min and then subjected to 22.5 min of global ischemia followed by 45 min reperfusion. Heart rate, ischemic contracture, end diastolic pressure, and developed ventricular pressure were monitored. At the completion of the experiment, hearts were homogenized and tissue extracts were assayed for glutathione peroxidase (GSH-Px) and thioredoxin reductase (Thx-Red) activity. Sodium selenite, at a concentration of 0.5 μM, demonstrated a protective effect on the recovery of cardiac function following I-R, as evidenced by a lower end diastolic pressure and enhanced recovery of rate pressure product. There was no beneficial effect observed in hearts perfused with 0.1 μM sodium selenite-supplemented buffer, whereas poorer functional recovery was observed in hearts perfused with 10 μM sodium selenite-supplemented buffer. The beneficial effect of sodium selenite was not mediated through increased activity of GSH-Px or Thx-Red. This study demonstrates that the addition of sodium selenite to reperfusion solutions, at an optimal concentration of 0.5 μM, assists in cardiac recovery following ischemia reperfusion.
Language eng
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006 by Humana Press Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048223

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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