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Validation of the aceto-carmine technique for evaluating phenotypic sex in Nile tilapia (Oreochromis niloticus) fry

Wassermann, Gustavo Javier and Afonso, Luis Orlando Bertolla 2002, Validation of the aceto-carmine technique for evaluating phenotypic sex in Nile tilapia (Oreochromis niloticus) fry, Ciência rural, vol. 32, no. 1, pp. 133-139.

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Title Validation of the aceto-carmine technique for evaluating phenotypic sex in Nile tilapia (Oreochromis niloticus) fry
Formatted title Validation of the aceto-carmine technique for evaluating phenotypic sex in Nile tilapia (Oreochromis niloticus) fry
Author(s) Wassermann, Gustavo Javier
Afonso, Luis Orlando Bertolla
Journal name Ciência rural
Volume number 32
Issue number 1
Start page 133
End page 139
Total pages 7
Publisher Centro
Place of publication Santa Maria, Brasil
Publication date 2002
ISSN 0103-8478
Keyword(s) sex evaluation
Nile tilapia
Summary In this study a method for evaluating phenotypic sex in Nile tilapia was validated. A technique that uses aceto-carmine squash mounts to stain the entire gonadal tissue for microscopic examination (Method 2- squash mounts) was compared with a technique based on traditional histology. Approximately 2600 Nile tilapia fry weighing and measuring, respectively, between 0.25-2.50g and 26-53mm, aged 35 to 60 days after hatch (DAH), were sexed using this methodology. In situ microscopic examination on the gonads was also performed. A reliable sexing using squash mount was possible with fish weighing more than 0.500g, 45-47 DAH. Results from microscopic observation using the aceto-carmine stain coincided 100% with the histological examination. Male gonadal tissue was characterized by the presence of cysts containing spermatogonia and spermatocytes, while females were easily identified by the presence of oocytes at the perinucleolar stage. The technique proved to be efficient not only in terms of evaluating sex proportion in fish but also because it allows immediate evaluation of gonadal sex and demands less time and labour.
Language eng
Field of Research 070401 Aquaculture
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2002, Centro
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048530

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.