Enzymatic synthesis of isopropyl acetate catalysed by immobilized bacillus cereus lipase in organic medium

Verma, Madan Lal, Azmi, Wamik and Kanwar, Shamsher Singh 2011, Enzymatic synthesis of isopropyl acetate catalysed by immobilized bacillus cereus lipase in organic medium, Enzyme research, vol. 2011, Article ID 919386, pp. 1-7.

Attached Files
Name Description MIMEType Size Downloads

Title Enzymatic synthesis of isopropyl acetate catalysed by immobilized bacillus cereus lipase in organic medium
Author(s) Verma, Madan Lal
Azmi, Wamik
Kanwar, Shamsher Singh
Journal name Enzyme research
Volume number 2011
Season Article ID 919386
Start page 1
End page 7
Total pages 7
Publisher Hindawi Publishing Corporation
Place of publication New York, N. Y.
Publication date 2011
ISSN 2090-0406
2090-0414
Keyword(s) fatty acid
isopropanol
acetic acid
lipase
Summary Selective production of fragrance fatty acid ester from isopropanol and acetic acid has been achieved using silica-immobilizedlipase of Bacillus cereus MTCC 8372. A purified thermoalkalophilic extracellular lipase was immobilized by adsorption onto the silica. The effects of various parameters like molar ratio of substrates (isopropanol and acetic acid; 25 to 100 mM), concentration ofbiocatalyst (25–125 mg/mL), reaction time, reaction temperature, organic solvents,molecular sieves, and initial water activity werestudied for optimal ester synthesis. Under optimized conditions, 66.0mM of isopropyl acetate was produced when isopropanol and acetic acid were used at 100mM: 75mM in 9h at 55◦C in n-heptane under continuous shaking (160 rpm) using bound lipase(25mg). Addition of molecular sieves (3 °A ×1.5mm) resulted in a marked increase in ester synthesis (73.0mM). Ester synthesiswas enhanced by water activity associated with pre-equilibrated saturated salt solution of LiCl. The immobilized lipase retained more than 50% of its activity after the 6th cycle of reuse.
Language eng
Field of Research 090899 Food Sciences not elsewhere classified
Socio Economic Objective 860106 Oils and Fats (incl. Margarines)
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2011, Madan Lal Verma et al.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048579

Document type: Journal Article
Collection: School of Life and Environmental Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Access Statistics: 67 Abstract Views, 2 File Downloads  -  Detailed Statistics
Created: Tue, 25 Sep 2012, 14:38:38 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.