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MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in arabidopsis

Cevik, Volkan, Kidd, Brendan N., Zhang, Peijun, Hill, Claire, Kiddle, Steve, Denby, Katherine J., Holub, Eric B., Cahill, David M., Manners, John M., Schenk, Peer M., Beynon, Jim and Kazan, Kemal 2012, MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in arabidopsis, Plant physiology, vol. 160, no. 1, pp. 541-555.

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Title MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in arabidopsis
Author(s) Cevik, Volkan
Kidd, Brendan N.
Zhang, Peijun
Hill, Claire
Kiddle, Steve
Denby, Katherine J.
Holub, Eric B.
Cahill, David M.
Manners, John M.
Schenk, Peer M.
Beynon, Jim
Kazan, Kemal
Journal name Plant physiology
Volume number 160
Issue number 1
Start page 541
End page 555
Total pages 15
Publisher American Society of Plant Biologists
Place of publication Rockville, Md.
Publication date 2012-09
ISSN 0032-0889
1532-2548
Summary The PHYTOCHROME AND FLOWERING TIME1 gene encoding the MEDIATOR25 (MED25) subunit of the eukaryotic Mediator complex is a positive regulator of jasmonate (JA)-responsive gene expression in Arabidopsis (Arabidopsis thaliana). Based on the function of the Mediator complex as a bridge between DNA-bound transcriptional activators and the RNA polymerase II complex, MED25 has been hypothesized to function in association with transcriptional regulators of the JA pathway. However, it is currently not known mechanistically how MED25 functions to regulate JA-responsive gene expression. In this study, we show that MED25 physically interacts with several key transcriptional regulators of the JA signaling pathway, including the APETALA2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) transcription factors OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERF59 and ERF1 as well as the master regulator MYC2. Physical interaction detected between MED25 and four group IX AP2/ERF transcription factors was shown to require the activator interaction domain of MED25 as well as the recently discovered Conserved Motif IX-1/EDLL transcription activation motif of MED25-interacting AP2/ERFs. Using transcriptional activation experiments, we also show that OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERF59- and ERF1-dependent activation of PLANT DEFENSIN1.2 as well as MYC2-dependent activation of VEGETATIVE STORAGE PROTEIN1 requires a functional MED25. In addition, MED25 is required for MYC2-dependent repression of pathogen defense genes. These results suggest an important role for MED25 as an integrative hub within the Mediator complex during the regulation of JA-associated gene expression.
Language eng
Field of Research 060705 Plant Physiology
060704 Plant Pathology
060702 Plant Cell and Molecular Biology
Socio Economic Objective 960413 Control of Plant Pests, Diseases and Exotic Species in Farmland, Arable Cropland and Permanent Cropland
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2012, American Society of Plant Biologists
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048804

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.