Isolation and characterisation via 454 sequencing of microsatellites from the tawny frogmouth, Podargus strigoides (Class Aves, Family Podargidae)

Hogan, Fiona E., Weaving, Marian, Johnston, Gregory R. and Gardner, Michael G. 2012, Isolation and characterisation via 454 sequencing of microsatellites from the tawny frogmouth, Podargus strigoides (Class Aves, Family Podargidae), Australian journal of zoology, vol. 60, no. 2, pp. 133-136.

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Title Isolation and characterisation via 454 sequencing of microsatellites from the tawny frogmouth, Podargus strigoides (Class Aves, Family Podargidae)
Author(s) Hogan, Fiona E.
Weaving, Marian
Johnston, Gregory R.
Gardner, Michael G.
Journal name Australian journal of zoology
Volume number 60
Issue number 2
Start page 133
End page 136
Total pages 4
Publisher CSIRO Publishing
Place of publication Collingwood, Vic
Publication date 2012-09-17
ISSN 0004-959X
1446-5698
Keyword(s) 454 GS-FLX
nocturnal
shotgun sequencing
urban
Summary We isolated 24 novel polymorphic microsatellite markers from the tawny frogmouth, a nocturnal bird endemic to Australia, which has successfully adapted to urban environments. Initially, 454 shotgun sequencing was used to identify 733 loci with primers designed. Of these, we trialled 30 in the target species of which all amplified a product of expected size. Subsequently, all 30 of these loci were screened for variation in 25 individuals, from a single population in Melbourne, Victoria, Australia. Twenty-eight loci were polymorphic with observed heterozygosity ranging from 0.03 to 0.96 (mean 0.58) and the number of alleles per locus ranged from 2 to 18 (average of 6.5); we confirmed that 24 loci conformed to Hardy–Weinberg expectations. The 24 loci identified here will be sufficient to unequivocally identify individuals and will be useful in understanding the reproductive ecology, population genetics and the gene flow amongst localities in urban environments where this bird thrives.

Language eng
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2012, CSIRO
Persistent URL http://hdl.handle.net/10536/DRO/DU:30049666

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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