Membrane nanotubes in myeloid cells in the adult mouse cornea represent a novel mode of immune cell interaction

Seyed-Razavi, Yashar, Hickey, Michael J., Kuffova, Lucia, McMenamin, Paul G. and Chinnery, Holly R. 2013, Membrane nanotubes in myeloid cells in the adult mouse cornea represent a novel mode of immune cell interaction, Immunology and cell biology, vol. 91, no. 1, pp. 89-95, doi: 10.1038/icb.2012.52.

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Title Membrane nanotubes in myeloid cells in the adult mouse cornea represent a novel mode of immune cell interaction
Author(s) Seyed-Razavi, Yashar
Hickey, Michael J.
Kuffova, Lucia
McMenamin, Paul G.
Chinnery, Holly R.
Journal name Immunology and cell biology
Volume number 91
Issue number 1
Start page 89
End page 95
Total pages 7
Publisher Nature Publishing Group
Place of publication London, England
Publication date 2013-01
ISSN 0818-9641
Keyword(s) CD11c
intercellular communication
dendritic cells
corneal inflammation
Summary Membrane nanotubes (MNTs) are newly discovered cellular extensions that are either blind-ended or can connect widely separated cells. They have predominantly been investigated in cultured isolated cells, however, previously we were the first group to demonstrate the existence of these structures in vivo in intact mammalian tissues. We previously demonstrated the frequency of both cell–cell or bridging MNTs and blind-ended MNTs was greatest between major histocompatibility complex (MHC) class II+ cells during corneal injury or TLR ligand-mediated inflammation. The present study aimed to further explore the dynamics of MNT formation and their size, presence in another tissue, the dura mater, and response to stress factors and an active local viral infection of the murine cornea. Confocal live cell imaging of myeloid-derived cells in inflamed corneal explants from Cx3cr1GFP and CD11ceYFP transgenic mice revealed that MNTs form de novo at a rate of 15.5 μm/min. This observation contrasts with previous studies that demonstrated that in vitro these structures originate from cell–cell contacts. Conditions that promote formation of MNTs include inflammation in vivo and cell stress due to serum starvation ex vivo. Herpes simplex virus-1 infection did not cause a significant increase in MNT numbers in myeloid cells in the cornea above that observed in injury controls, confirming that corneal epithelium injury alone elicits MNT formation in vivo. These novel observations extend the currently limited understanding of MNTs in live mammalian tissues.
Language eng
DOI 10.1038/icb.2012.52
Field of Research 110707 Innate Immunity
Socio Economic Objective 920108 Immune System and Allergy
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2013, Nature Publishing Group
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Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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Created: Mon, 14 Jan 2013, 13:47:45 EST by Jane Moschetti

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