Systematic investigation of oxygen and growth factors in clinically valid ex vivo expansion of cord blood CD34+ hematopoietic progenitor cells

Tursky, Melinda L., Collier, Fiona M., Ward, Alister C. and Kirkland, Mark A. 2012, Systematic investigation of oxygen and growth factors in clinically valid ex vivo expansion of cord blood CD34+ hematopoietic progenitor cells, Cytotherapy, vol. 14, no. 6, pp. 679-685.

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Title Systematic investigation of oxygen and growth factors in clinically valid ex vivo expansion of cord blood CD34+ hematopoietic progenitor cells
Author(s) Tursky, Melinda L.
Collier, Fiona M.
Ward, Alister C.
Kirkland, Mark A.
Journal name Cytotherapy
Volume number 14
Issue number 6
Start page 679
End page 685
Total pages 7
Publisher Elsevier
Place of publication Oxford, England
Publication date 2012-07
ISSN 1465-3249
1477-2566
Keyword(s) cord blood
ex vivo expansion
growth factors
hematopoiesis
hematopoietic progenitor cells
oxygen
stem cell transplantation
Summary Background aims. Cord blood is considered to be a superior source of hematopoietic stem and progenitor cells for transplantation, but clinical use is limited primarily because of the low numbers of cells harvested. Ex vivo expansion has the potential to provide a safe, effective means of increasing cell numbers. However, an absence of consensus regarding optimum expansion conditions prevents standard implementation. Many studies lack clinical applicability, or have failed to investigate the combinational effects of different parameters.

Methods. This is the first study to characterize systematically the effect of growth factor combinations across multiple oxygen levels on the ex vivo expansion of cord blood CD34 hematopoietic cells utilizing clinically approvable reagents and methodologies throughout.

Results. Optimal fold expansion, as assessed both phenotypically and functionally, was greatest with thrombopoietin, stem cell factor, Flt-3 ligand and interleukin-6 at an oxygen level of 10%. With these conditions, serial expansion showed continual target population expansion and consistently higher expression levels of self-renewal associated genes.

Conclusions. This study has identified optimized fold expansion conditions, with the potential for direct clinical translation to increase transplantable cell dose and as a baseline methodology against which future factors can be tested.
Language eng
Field of Research 110202 Haematology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30050497

Document type: Journal Article
Collections: School of Medicine
Alfred Deakin Research Institute
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