Fructose containing sugars modulate mRNA of lipogenic genes ACC and FAS and protein levels of transcription factors ChREBP and SREBPIc with no effect on body weight or liver fat

Janevski, Mile, Ratnayake, Sunil, Siljanovski, Svetlana, McGlynn, Maree A., Cameron-Smith, David and Lewandowski, Paul 2012, Fructose containing sugars modulate mRNA of lipogenic genes ACC and FAS and protein levels of transcription factors ChREBP and SREBPIc with no effect on body weight or liver fat, Food and function, vol. 3, no. 2, pp. 141-149.

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Title Fructose containing sugars modulate mRNA of lipogenic genes ACC and FAS and protein levels of transcription factors ChREBP and SREBPIc with no effect on body weight or liver fat
Author(s) Janevski, Mile
Ratnayake, Sunil
Siljanovski, Svetlana
McGlynn, Maree A.
Cameron-Smith, David
Lewandowski, Paul
Journal name Food and function
Volume number 3
Issue number 2
Start page 141
End page 149
Total pages 9
Publisher R S C Publications
Place of publication Cambridge, England
Publication date 2012-02
ISSN 2042-6496
2042-650X
Keyword(s) body weight
fat contents
fat metabolism
fat oxidation
gene transcripts
liver lipids
MRNA level
nuclear fractions
on-body
protein level
transcriptional regulator
transport proteins
weight gain
Summary The aim of this study was to determine the effects of high-glucose, high-fructose and high-sucrose diets on weight gain, liver lipid metabolism and gene expression of proteins involved with hepatic fat metabolism. Rats were fed a diet containing either 60% glucose, 60% fructose, 60% sucrose, or a standard chow for 28 days. Results indicated that high-fructose and high-sucrose diets were associated with higher mRNA levels of gene transcripts involved with fat synthesis; ACC, FAS and ChREBP, with no change in SREBP-1C mRNA. The protein level of ChREBP and SREBP1c was similar in liver homogenates from all groups, but were higher in nuclear fractions from the liver of high-fructose and high-sucrose fed rats. The mRNA level of gene transcripts involved with fat oxidation was the same in all three diets, whilst a high-fructose diet was associated with greater amount of mRNA of the fat transporter CD36. Despite the changes in mRNA of lipogenic proteins, the body weight of animals from each group was the same and the livers from rats fed high-fructose and high-sucrose diets did not contain more fat than control diet livers. In conclusion, changing the composition of the principal monosaccharide in the diet to a fructose containing sugar elicits changes in the level of hepatic mRNA of lipogenic and fat transport proteins and protein levels of their transcriptional regulators; however this is not associated with any changes in body weight or liver fat content.
Language eng
Field of Research 111103 Nutritional Physiology
Socio Economic Objective 920411 Nutrition
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30050513

Document type: Journal Article
Collection: School of Medicine
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