Orally administered [14C]DPA and [14C]DHA are metabolised differently to [14C]EPA in rats

Kaur, Gunveen, Molero, Juan C., Weisinger, Harrison S. and Sinclair, Andrew J. 2013, Orally administered [14C]DPA and [14C]DHA are metabolised differently to [14C]EPA in rats, British journal of nutrition, vol. 109, no. 3, pp. 441-448.

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Title Orally administered [14C]DPA and [14C]DHA are metabolised differently to [14C]EPA in rats
Author(s) Kaur, Gunveen
Molero, Juan C.
Weisinger, Harrison S.
Sinclair, Andrew J.
Journal name British journal of nutrition
Volume number 109
Issue number 3
Start page 441
End page 448
Total pages 8
Publisher Cambridge University Press
Place of publication Cambridge, England
Publication date 2013-02
ISSN 0007-1145
1475-2662
Keyword(s) n-3 PUFA
docosapentaenoic acid
EPA
DHA
b-oxidation
Summary Previous studies have revealed that C20 PUFA are significantly less oxidised to CO2 in whole-body studies compared with SFA, MUFA and C18 PUFA. The present study determined the extent to which three long-chain PUFA, namely 20 : 5n-3 EPA, 22 : 5n-3 docosapentaenoic acid (DPA) and 22 : 6n-3 DHA, were catabolised to CO2 or, conversely, incorporated into tissue lipids. Rats were administered a single oral dose of 2·5 μCi [1-14C]DPA, [1-14C]EPA, [1-14C]DHA or [1-14C]oleic acid (18 : 1n-9; OA), and were placed in a metabolism chamber for 6 h where exhaled 14CO2 was trapped and counted for radioactivity. Rats were euthanised after 24 h and tissues were removed for analysis of radioactivity in tissue lipids. The results showed that DPA and DHA were catabolised to CO2 significantly less compared with EPA and OA (P< 0·05). The phospholipid (PL) fraction was the most labelled for all three n-3 PUFA compared with OA in all tissues, and there was no difference between C20 and C22 n-3 PUFA in the proportion of label in the PL fraction. The DHA and DPA groups showed significantly more label than the EPA group in both skeletal muscle and heart. In the brain and heart tissue, there was significantly less label in the cholesterol fraction from the C22 n-3 PUFA group compared with the C20 n-3 PUFA group. The higher incorporation of DHA and DPA into the heart and skeletal muscle, compared with EPA, suggests that these C22 n-3 PUFA might play an important role in these tissues.
Language eng
Field of Research 111103 Nutritional Physiology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2012, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30050693

Document type: Journal Article
Collection: School of Medicine
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