Enhanced cytotoxic function of natural killer and natural killer T-like cells associated with decreased CD94 (Kp43) in the chronic obstructive pulmonary disease airway

Hodge, Greg, Mukaro, Violet, Holmes, Mark, Reynolds, Paul N. and Hodge, Sandra 2013, Enhanced cytotoxic function of natural killer and natural killer T-like cells associated with decreased CD94 (Kp43) in the chronic obstructive pulmonary disease airway, Respirology, vol. 18, no. 2, pp. 369-376.

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Title Enhanced cytotoxic function of natural killer and natural killer T-like cells associated with decreased CD94 (Kp43) in the chronic obstructive pulmonary disease airway
Author(s) Hodge, Greg
Mukaro, Violet
Holmes, Mark
Reynolds, Paul N.
Hodge, Sandra
Journal name Respirology
Volume number 18
Issue number 2
Start page 369
End page 376
Total pages 8
Publisher Wiley-Blackwell Publishing Asia
Place of publication Richmond, Vic.
Publication date 2013-02
ISSN 1323-7799
Keyword(s) bronchoalveolar lavage fluid
natural killer and natural killer T-like cells
cytotoxicity
chronic obstructive pulmonary disease
CD94 (Kp43)
Summary Background and objective: Natural killer (NK) and natural killer T (NKT)-like cells represent a small but important proportion of effector lymphocytes that we have previously shown to be major sources of pro-inflammatory cytokines and granzymes. We hypothesized that these cells would be increased in the airway in chronic obstructive pulmonary disease (COPD), accompanied by reduced expression of the inhibitory receptor CD94 (Kp43) and increased expression of cytotoxic mediators granzyme B and perforin.
Methods: We measured NK and NKT-like cells and their expression of CD94 in the blood of COPD patients (n = 71; 30 current and 41 ex-smokers), smokers (16) and healthy controls (25), and bronchoalveolar lavage fluid (BALF) from a cohort of subjects (19 controls, 12 smokers, 33 COPD). Activation was assessed by measuring CD69 in blood and the cytotoxic potential of NK cells by measuring granzymes A and B, and using a cytotoxicity assay in blood and BALF.
Results: In blood in COPD, there were no significant changes in the proportion of NK or NKT-like cells or expression of granzyme A or NK cytotoxic potential versus controls. There was, however, increased expression of granzyme B and decreased expression of CD94 by both cell types versus controls. The proportion of NK and NKT-like cells were increased in BALF in COPD, associated with increased NK cytotoxicity, increased expression of granzyme B and decreased expression of the inhibitory receptor CD94 by both cell types.
Conclusions: Treatment strategies that target NK and NKT-like cells, their cytotoxicity and production of inflammatory mediators in the airway may improve COPD morbidity.
Language eng
Field of Research 110299 Cardiovascular Medicine and Haematology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30050708

Document type: Journal Article
Collection: School of Medicine
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Created: Tue, 19 Feb 2013, 14:38:08 EST by Jane Moschetti

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