Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation

Kohzaki, Kenichi, Vingrys, Algis J., Armitage, James A. and Bui, Bang V. 2013, Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation, Basic science, vol. 251, no. 2, pp. 529-535.

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Title Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation
Author(s) Kohzaki, Kenichi
Vingrys, Algis J.
Armitage, James A.
Bui, Bang V.
Journal name Basic science
Volume number 251
Issue number 2
Start page 529
End page 535
Total pages 7
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 2013-02
ISSN 0721-832X
Keyword(s) Electroretinogram
Intraocular pressure
Diabetes
Hyperglycemia
Streptozotocin
Rat
Retina
Summary Background
We consider whether pre-existing streptozotocin induced hyperglycemia in rats affects the ability of the eye to cope with a single episode of acute intraocular pressure (IOP) elevation.
Methods
Electroretinogram (ERG) responses were measured (−6.08 to 1.92 log cd.s.m−2) in anaesthetized (60:5 mg/kg ketamine:xylazine) dark-adapted (>12 h) adult Sprague–Dawley rats 1 week after a single acute IOP elevation to 70 mmHg for 60 min. This was undertaken in rats treated 11 weeks earlier with streptozotocin (STZ, n = 12, 50 mg/kg at 6 weeks of age) or citrate buffer (n = 12). ERG responses were analyzed to derive an index of photoreceptor (a-wave), ON-bipolar (b-wave), amacrine (oscillatory potentials) and inner retinal (positive scotopic threshold response, pSTR) function.
Results
One week following acute IOP elevation there was a significant reduction of the ganglion cell pSTR (−35 ± 11 %, P = 0.0161) in STZ-injected animals. In contrast the pSTR in citrate-injected animals was not significant changed (+16 ± 14 %). The negative component of the STR was unaffected by IOP elevation in either citrate or STZ-treated groups. Photoreceptoral (a-wave, citrate-control +4 ± 3 %, STZ +4 ± 5 %) and ON-bipolar cell (b-wave, control +4 ± 3 %, STZ +4 ± 5 %) mediated responses were not significantly affected by IOP elevation in either citrate- or STZ-injected rats. Finally, oscillatory potentials (citrate-control +8 ± 23 %, STZ +1 ± 17 %) were not reduced 1 week after IOP challenge.
Conclusions
The ganglion cell dominated pSTR was reduced following a single episode of IOP elevation in STZ diabetic, but not control rats. These data indicate that hyperglycemia renders the inner retina more susceptible to IOP elevation.
Language eng
Field of Research 111303 Vision Science
Socio Economic Objective 920107 Hearing, Vision, Speech and Their Disorders
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30050742

Document type: Journal Article
Collection: School of Medicine
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