A fresh look at the validity of the diffusion approximation for modeling fluorescence spectroscopy in biological tissue
Handapangoda, Chinta Chamalie, Premaratne, Malin and Nahavandi, Saeid 2012, A fresh look at the validity of the diffusion approximation for modeling fluorescence spectroscopy in biological tissue, in EMBC 2012 : Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, IEEE, Piscataway, N.J., pp. 1494-1497, doi: 10.1109/EMBC.2012.6346224.
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A fresh look at the validity of the diffusion approximation for modeling fluorescence spectroscopy in biological tissue
Fluorescence has become a widely used technique for applications in noninvasive diagnostic tissue spectroscopy. The standard model used for characterizing fluorescence photon transport in biological tissue is based on the diffusion approximation. On the premise that the total energy of excitation and fluorescent photon flows must be conserved, we derive the widely used diffusion equations in fluorescence spectroscopy and show that there must be an additional term to account for the transport of fluorescent photons. The significance of this additional term in modeling fluorescence spectroscopy in biological tissue is assessed.
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