Structure and biodegradation mechanism of milled Bombyx mori silk particles

Rajkhowa, Rangam, Hu, Xiao, Tsuzuki, Takuya, Kaplan, David L. and Wang, Xungai 2012, Structure and biodegradation mechanism of milled Bombyx mori silk particles, Biomacromolecules, vol. 13, no. 8, pp. 2503-2512, doi: 10.1021/bm300736m.

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Title Structure and biodegradation mechanism of milled Bombyx mori silk particles
Author(s) Rajkhowa, RangamORCID iD for Rajkhowa, Rangam
Hu, Xiao
Tsuzuki, Takuya
Kaplan, David L.
Wang, XungaiORCID iD for Wang, Xungai
Journal name Biomacromolecules
Volume number 13
Issue number 8
Start page 2503
End page 2512
Total pages 10
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2012
ISSN 1525-7797
Summary The aim of this study was to understand the structure and biodegradation relationships of silk particles intended for targeted biomedical applications. Such a study is also useful in understanding structural remodelling of silk debris that may be generated from silk-based implants. Ultrafine silk particles were prepared using a combination of efficient wet-milling and spray-drying processes with no addition of chemicals other than those used in degumming. Milling reduced the intermolecular stacking forces within the β-sheet crystallites without changing the intramolecular binding energy. Because of the rough morphology and the ultrafine size of the particles, degradation of silk particles by protease XIV was increased by about 3-fold compared to silk fibers. Upon biodegradation, the thermal degradation temperature of silk increased, which was attributed to the formation of tight aggregates by the hydrolyzed residual macromolecules. A model of the biodegradation mechanism of silk particles was developed based on the experimental data. The model explains the process of disintegration of β-sheets, supported by quantitative secondary structural analysis and microscopic images. © 2012 American Chemical Society.
Language eng
DOI 10.1021/bm300736m
Field of Research 090301 Biomaterials
091299 Materials Engineering not elsewhere classified
Socio Economic Objective 860403 Natural Fibres, Yarns and Fabrics
HERDC Research category C1 Refereed article in a scholarly journal
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