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Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc)

Sugumar, D., Kong, L.X., Ismail, Asma, Ravichandran, M. and Yin, Lee Su 2012, Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc), Biomicrofluidics, vol. 6, no. 1, pp. 1-13.

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Title Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc)
Author(s) Sugumar, D.
Kong, L.X.
Ismail, Asma
Ravichandran, M.
Yin, Lee Su
Journal name Biomicrofluidics
Volume number 6
Issue number 1
Start page 1
End page 13
Total pages 13
Publisher American Institute of Physics
Place of publication Melville, N.Y.
Publication date 2012
ISSN 1932-1058
Keyword(s) chip
PCR
microchip
Summary Multiple sample DNA amplification was done by using a novel rotary-linear motion polymerase chain reaction (PCR) device. A simple compact disc was used to create the stationary sample chambers which are individually temperature controlled. The PCR was performed by shuttling the samples to different temperature zones by using a combined rotary-linear movement of the disc. The device was successfully used to amplify up to 12 samples in less than 30 min with a sample volume of 5 μl. A simple spring loaded heater mechanism was introduced to enable good thermal contact between the samples and the heaters. Each of the heater temperatures are controlled by using a simple proportional–integral–derivative pulse width modulation control system. The results show a good improvement in the amplification rate and duration of the samples. The reagent volume used was reduced to nearly 25% of that used in conventional method.
Language eng
Field of Research 109999 Technology not elsewhere classified
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category C2 Other contribution to refereed journal
Copyright notice ©2012, American Institute of Physics
Persistent URL http://hdl.handle.net/10536/DRO/DU:30051199

Document type: Journal Article
Collections: Institute for Frontier Materials
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.