Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc)
Sugumar, D., Kong, L.X., Ismail, Asma, Ravichandran, M. and Yin, Lee Su 2012, Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc), Biomicrofluidics, vol. 6, no. 1, pp. 1-13.
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Multiple sample DNA amplification was done by using a novel rotary-linear motion polymerase chain reaction (PCR) device. A simple compact disc was used to create the stationary sample chambers which are individually temperature controlled. The PCR was performed by shuttling the samples to different temperature zones by using a combined rotary-linear movement of the disc. The device was successfully used to amplify up to 12 samples in less than 30 min with a sample volume of 5 μl. A simple spring loaded heater mechanism was introduced to enable good thermal contact between the samples and the heaters. Each of the heater temperatures are controlled by using a simple proportional–integral–derivative pulse width modulation control system. The results show a good improvement in the amplification rate and duration of the samples. The reagent volume used was reduced to nearly 25% of that used in conventional method.
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