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Azide photochemistry for facile modification of graphitic surfaces : preparation of DNA-coated carbon nanotubes for biosensing

Moghaddam, Minoo J., Yang, Wenrong, Bojarski, Barbara, Gengenbach, Thomas R., Gao, Mei, Zareie, Hai and McCall, Maxine J. 2012, Azide photochemistry for facile modification of graphitic surfaces : preparation of DNA-coated carbon nanotubes for biosensing, Nanotechnology, vol. 23, no. 42, pp. 1-12, doi: 10.1088/0957-4484/23/42/425503.

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Title Azide photochemistry for facile modification of graphitic surfaces : preparation of DNA-coated carbon nanotubes for biosensing
Author(s) Moghaddam, Minoo J.
Yang, WenrongORCID iD for Yang, Wenrong
Bojarski, Barbara
Gengenbach, Thomas R.
Gao, Mei
Zareie, Hai
McCall, Maxine J.
Journal name Nanotechnology
Volume number 23
Issue number 42
Start page 1
End page 12
Total pages 12
Publisher IOP Publishing
Place of publication Bristol, England
Publication date 2012
ISSN 0957-4484
Summary A facile, two-step method for chemically attaching single-stranded DNA to graphitic surfaces, represented here by carbon nanotubes, is reported. In the first step, an azide-containing compound, N-5-azido-nitrobenzoyloxy succinimide (ANB-NOS), is used to form photo-adducts on the graphitic surfaces in a solid-state photochemical reaction, resulting in active ester groups being oriented for the subsequent reactions. In the second step, pre-synthesized DNA strands bearing a terminal amine group are coupled in an aqueous solution with the active esters on the photo-adducts. The versatility of the method is demonstrated by attaching pre-synthesized DNA to surfaces of carbon nanotubes in two platforms—as vertically-aligned multi-walled carbon nanotubes on a solid support and as tangled single-walled carbon nanotubes in mats. The reaction products at various stages were characterized by x-ray photoelectron spectroscopy. Two different assays were used to check that the DNA strands attached to the carbon nanotubes were able to bind their partner strands with complementary base sequences. The first assay, using partner DNA strands tethered to gold nanoparticles, enabled the sites of DNA attachment to the carbon nanotubes to be identified in TEM images. The second assay, using radioactively labelled partner DNA strands, quantified the density of functional DNA strands attached to the carbon nanotubes. The diversity of potential applications for these DNA-modified carbon-nanotube platforms is exemplified here by the successful use of a DNA-modified single-walled carbon-nanotube mat as an electrode for the specific detection of metal ions.
Language eng
DOI 10.1088/0957-4484/23/42/425503
Field of Research 100708 Nanomaterials
030306 Synthesis of Materials
030301 Chemical Characterisation of Materials
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
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Document type: Journal Article
Collection: School of Life and Environmental Sciences
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