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Bone marrow chimeric mice reveal a role for CX3CR1 in maintenance of the monocyte-derived cell population in the olfactory neuroepithelium

Vukovic, Jana, Blomster, Linda V., Chinnery, Holly R., Weninger, Wolfgang, Jung, Steffen, McMenamin, Paul G. and Ruitenberg, Marc J. 2010, Bone marrow chimeric mice reveal a role for CX3CR1 in maintenance of the monocyte-derived cell population in the olfactory neuroepithelium, Journal of leukocyte biology, vol. 88, no. 4, pp. 645-654, doi: 10.1189/jlb.0410194.

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Title Bone marrow chimeric mice reveal a role for CX3CR1 in maintenance of the monocyte-derived cell population in the olfactory neuroepithelium
Author(s) Vukovic, Jana
Blomster, Linda V.
Chinnery, Holly R.
Weninger, Wolfgang
Jung, Steffen
McMenamin, Paul G.
Ruitenberg, Marc J.
Journal name Journal of leukocyte biology
Volume number 88
Issue number 4
Start page 645
End page 654
Total pages 10
Publisher Federation of American Societies for Experimental Biology
Place of publication Bethesda, Md.
Publication date 2010
ISSN 0741-5400
Keyword(s) macrophages
dendritic cells
irradiation
neurogenesis
CD11c
chemokine
Summary Macrophages in the olfactory neuroepithelium are thought to play major roles in tissue homeostasis and repair. However, little information is available at present about possible heterogeneity of these monocyte-derived cells, their turnover rates, and the role of chemokine receptors in this process. To start addressing these issues, this study used Cx3cr1gfp mice, in which the gene sequence for eGFP was knocked into the CX3CR1 gene locus in the mutant allele. Using neuroepithelial whole-mounts from Cx3cr1gfp/+ mice, we show that eGFP+ cells of monocytic origin are distributed in a loose network throughout this tissue and can be subdivided further into two immunophenotypically distinct subsets based on MHC-II glycoprotein expression. BM chimeric mice were created using Cx3cr1gfp/+ donors to investigate turnover of macrophages (and other monocyte-derived cells) in the olfactory neuroepithelium. Our data indicate that the monocyte-derived cell population in the olfactory neuroepithelium is actively replenished by circulating monocytes and under the experimental conditions, completely turned over within 6 months. Transplantation of Cx3cr1gfp/gfp (i.e., CX3CR1-deficient) BM partially impaired the replenishment process and resulted in an overall decline of the total monocyte-derived cell number in the olfactory epithelium. Interestingly, replenishment of the CD68lowMHC-II+ subset appeared minimally affected by CX3CR1 deficiency. Taken together, the established baseline data about heterogeneity of monocyte-derived cells, their replenishment rates, and the role of CX3CR1 provide a solid basis to further examine the importance of different monocyte subsets for neuroregeneration at this unique frontier with the external environment.
Language eng
DOI 10.1189/jlb.0410194
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2010, Federation of American Societies for Experimental Biology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30052123

Document type: Journal Article
Collection: School of Medicine
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