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Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice

Chinnery, Holly R., Ruitenberg, Marc J. and McMenamin, Paul G. 2010, Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice, Journal of neuropathology and experimental neurology, vol. 69, no. 9, pp. 896-909.

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Title Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice
Author(s) Chinnery, Holly R.
Ruitenberg, Marc J.
McMenamin, Paul G.
Journal name Journal of neuropathology and experimental neurology
Volume number 69
Issue number 9
Start page 896
End page 909
Total pages 14
Publisher Association of the Journal of Neuropathology and Experimental Neurology
Place of publication New York, N.Y.
Publication date 2010
ISSN 0022-3069
Keyword(s) brain
chemokines
dendritic cells
dura mater
macrophages
pia mater
turnover
Summary The mouse dura mater, pia mater, and choroid plexus contain resident macrophages and dendritic cells (DCs). These cells participate in immune surveillance, phagocytosis of cellular debris, uptake of antigens from the surrounding cerebrospinal fluid and immune regulation in many pathologic processes. We used Cx3cr1 knock-in, CD11c-eYFP transgenic and bone marrow chimeric mice to characterize the phenotype, density and replenishment rate of monocyte-derived cells in the meninges and choroid plexus and to assess the role of the chemokine receptor CX3CR1 on their number and tissue distribution. Iba-1 major histocompatibility complex (MHC) Class II CD169 CD68 macrophages and CD11c putative DCs were identified in meningeal and choroid plexus whole mounts. Comparison of homozygous and heterozygous Cx3cr1 mice did not reveal CX3CR1-dependancy on density, distribution or phenotype of monocyte-derived cells. In turnover studies, wild type lethally irradiated mice were reconstituted with Cx3cr1/-positive bone marrow and were analyzed at 3 days, 1, 2, 4 and 8 weeks after transplantation. There was a rapid replenishment of CX3CR1-positive cells in the dura mater (at 4 weeks) and the choroid plexus was fully reconstituted by 8 weeks. These data provide the foundation for future studies on the role of resident macrophages and DCs in conditions such as meningitis, autoimmune inflammatory disease and in therapies involving irradiation and hematopoietic or stem cell transplantation.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2010, Association of the Journal of Neuropathology and Experimental Neurology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30052124

Document type: Journal Article
Collection: School of Medicine
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