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Methods for the analysis of histone H3 and H4 acetylation in blood

Rigby, Lin, Muscat, Andrea, Ashley, David and Algar, Elizabeth 2012, Methods for the analysis of histone H3 and H4 acetylation in blood, Epigenetics, vol. 7, no. 8, pp. 875-882, doi: 10.4161/epi.20983.

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Title Methods for the analysis of histone H3 and H4 acetylation in blood
Author(s) Rigby, Lin
Muscat, Andrea
Ashley, David
Algar, Elizabeth
Journal name Epigenetics
Volume number 7
Issue number 8
Start page 875
End page 882
Total pages 8
Publisher Landes Bioscience
Place of publication Georgetown, Tex.
Publication date 2012
ISSN 1559-2308
1559-2294
Keyword(s) LBH589
histone acetylation
histone deacetylase inhibitors
paediatrics
panobinostat
Summary LBH589 is one of the many histone deacetylase inhibitors (HDACi) that are currently in clinical trial. Despite their wide-spread use, there is little literature available describing the typical levels of histone acetylation in untreated peripheral blood, the treatment and storage of samples to retain optimal measurement of histone acetylation nor methods by which histone acetylation analysis may be monitored and measured during the course of a patient’s treatment. In this study, we have used cord or peripheral blood as a source of human leukocytes, performed a comparative analysis of sample processing methods and developed a flow cytometric method suitable for monitoring histone acetylation in isolated lymphocytes and liquid tumors. Western blotting and immunohistochemistry techniques have also been addressed. We have tested these methods on blood samples collected from four patients treated with LBH589 as part of an Australian Children’s Cancer Clinical Trial (CLBH589AAU03T) and show comparable results when comparing in vitro and in vivo data. This paper does not seek to correlate histone acetylation levels in peripheral blood with clinical outcome but describes methods of analysis that will be of interest to clinicians and scientists monitoring the effects of HDACi on histone acetylation in blood samples in clinical trials or in related research studies.
Language eng
DOI 10.4161/epi.20983
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30052149

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.