Goyal, Arshi, Jain, Yachna, Verma, Madan Lal and Jana, Asim Kumar 2010, Fundamentals and applications of Maldi-Tof MS, in BEATS 2010 : Proceedings of the 2010 International Conference on Biomedical Engineering and Assistive Technologies, Jalandhar, India, BEATS, Jalandhar, India, pp. 1-4.
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MALDI was developed for the ionisation of relatively large polypeptides and proteins but its application has widened to incorporate glycoproteins, oligonucleotides and complex carbohydrates. A great advantage of MALDI TOF MS is that the process of soft-ionisation causes little or no fragmentation of analytes, allowing the molecular ions of analytes to be identified, even within mixtures. MALDI TOF MS analysis is sensitive and very rapid as once the sample has been mixed with a 'matrix' on a MALDI target, a spectrum can be generated within seconds. Hence, the majority of protein and oligonucleotide analysis in the context of high throughput proteomics and genomics is carried out by MALDI TOF mass spectrometry.
Field of Research
100301 Biocatalysis and Enzyme Technology 100306 Industrial Molecular Engineering of Nucleic Acids and Proteins 100703 Nanobiotechnology