Single cell stiffness measurement at various humidity conditions by nanomanipulation of a nano-needle

Shen, Yajing, Nakajima, Masahiro, Yang, Zhan, Tajima, Hirotaka, Najdovski, Zoran, Homma, Michio and Fukuda, Toshio 2013, Single cell stiffness measurement at various humidity conditions by nanomanipulation of a nano-needle, Nanotechnology, vol. 24, no. 14, pp. 1-9.

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Title Single cell stiffness measurement at various humidity conditions by nanomanipulation of a nano-needle
Author(s) Shen, Yajing
Nakajima, Masahiro
Yang, Zhan
Tajima, Hirotaka
Najdovski, Zoran
Homma, Michio
Fukuda, Toshio
Journal name Nanotechnology
Volume number 24
Issue number 14
Start page 1
End page 9
Total pages 9
Publisher IOP Press
Place of publication Bristol, England
Publication date 2013
ISSN 0957-4484
1361-6528
Summary This paper presents a method for single cell stiffness measurement based on a nano-needle and nanomanipulation. The nano-needle with a buffering beam was fabricated from an atomic force microscope cantilever by the focused ion beam etching technique. Wild type yeast cells (W303) were prepared and placed on the sample stage inside an environmental scanning electron microscope (ESEM) chamber. The nanomanipulator actuated the nano-needle to press against a single yeast cell. As a result, the deformation of the cell and nano-needle was observed by the ESEM system in real-time. Finally, the stiffness of the single cell was determined based on this deformation information. To reveal the relationship between the cell stiffness and the environmental humidity conditions, the cell stiffness was measured at three different humidity conditions, i.e. 40, 70 and 100%, respectively. The results show that the stiffness of a single cell is reduced with increasing humidity.
Language eng
Field of Research 089999 Information and Computing Sciences not elsewhere classified
Socio Economic Objective 970108 Expanding Knowledge in the Information and Computing Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30055343

Document type: Journal Article
Collection: Centre for Intelligent Systems Research
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