Cell biological responses of osteoblasts on anodized nanotubular surface of a titanium-zirconium alloy

Sista, Subhash, Nouri, Alireza, Li, Yuncang, Wen, Cuie, Hodgson, Peter D. and Pande, Gopal 2013, Cell biological responses of osteoblasts on anodized nanotubular surface of a titanium-zirconium alloy, Journal of biomedical materials research - Part A, vol. 101, no. 12, pp. 3416-3430, doi: 10.1002/jbm.a.34638.

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Title Cell biological responses of osteoblasts on anodized nanotubular surface of a titanium-zirconium alloy
Author(s) Sista, Subhash
Nouri, Alireza
Li, Yuncang
Wen, Cuie
Hodgson, Peter D.
Pande, Gopal
Journal name Journal of biomedical materials research - Part A
Volume number 101
Issue number 12
Start page 3416
End page 3430
Total pages 15
Publisher Wiley
Place of publication London, England
Publication date 2013
ISSN 1549-3296
Keyword(s) anodization
titanium alloy
gene expression
Summary Anodization of titanium and its alloys, under controlled conditions, generates a nanotubular architecture on the material surface. The biological consequences of such changes are poorly understood, and therefore, we have analyzed the cellular and molecular responses of osteoblasts that were plated on nanotubular anodized surface of a titanium-zirconium (TiZr) alloy. Upon comparing these results with those obtained on acid etched and polished surfaces of the same alloy, we observed a significant increase in adhesion and proliferation of cells on anodized surfaces as compared to acid etched or polished surface. The expression of genes related to cell adhesion was high only on anodized TiZr, but that of genes related to osteoblast differentiation and osteocalcin protein and extracellular matrix secretion were higher on both anodized and acid etched surfaces. Examination of surface morphology, topography, roughness, surface area and wettability using scanning electron microscopy, atomic force microscopy, and contact angle goniometry, showed that higher surface area, hydrophilicity, and nanoscale roughness of nanotubular TiZr surfaces, which were generated specifically by the anodization process, could strongly enhance the adhesion and proliferation of osteoblasts. We propose that biological properties of known bioactive titanium alloys can be further enhanced by generating nanotubular surfaces using anodization.
Language eng
DOI 10.1002/jbm.a.34638
Field of Research 060106 Cellular Interactions (incl Adhesion, Matrix, Cell Wall)
090301 Biomaterials
100706 Nanofabrication, Growth and Self Assembly
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30055349

Document type: Journal Article
Collections: Institute for Frontier Materials
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