Monitoring the early stage self-assembly of enzyme-assisted peptide hydrogels

Williams, Richard J., Gardiner, James, Sorensen, Anders B., Marchesan, Silvia, Mulder, Roger J., McLean, Keith M. and Hartley, Patrick G. 2013, Monitoring the early stage self-assembly of enzyme-assisted peptide hydrogels, Australian journal of chemistry, vol. 66, no. 5, pp. 572-578, doi: 10.1071/CH12557.

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Title Monitoring the early stage self-assembly of enzyme-assisted peptide hydrogels
Author(s) Williams, Richard J.
Gardiner, James
Sorensen, Anders B.
Marchesan, Silvia
Mulder, Roger J.
McLean, Keith M.
Hartley, Patrick G.
Journal name Australian journal of chemistry
Volume number 66
Issue number 5
Start page 572
End page 578
Total pages 7
Publisher CSIRO Publishing
Place of publication Melbourne, Vic.
Publication date 2013
ISSN 0004-9425
Summary The early stages of the self-assembly of peptide hydrogels largely determine their final material properties. Here we discuss experimental methodologies for monitoring the self-assembly kinetics which underpin peptide hydrogel formation. The early stage assembly of an enzyme-catalysed Fmoc-trileucine based self-assembled hydrogel was examined using spectroscopic techniques (circular dichroism, CD, and solution NMR) as well as chromatographic (HPLC) and mechanical (rheology) techniques. Optimal conditions for enzyme-assisted hydrogel formation were identified and the kinetics examined. A lag time associated with the formation and accumulation of the self-assembling peptide monomer was observed and a minimum hydrogelator concentration required for gelation was identified. Subsequent formation of well defined nano-and microscale structures lead to self-supporting hydrogels at a range of substrate and enzyme concentrations. 1H NMR monitoring of the early self-assembly process revealed trends that were well in agreement with those identified using traditional methods (i.e. HPLC, CD, rheology) demonstrating 1H NMR spectroscopy can be used to non-invasively monitor the self-assembly of peptide hydrogels without damaging or perturbing the system.
Language eng
DOI 10.1071/CH12557
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1 Refereed article in a scholarly journal
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Document type: Journal Article
Collection: School of Life and Environmental Sciences
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