Identification and functional characterization of a novel monotreme - specific antibacterial protein expressed during lactation

Bisana, Swathi, Kumar, Satish, Rismiller, Peggy, Nicol, Stewart C., Lefevre, Christophe, Nicholas, Kevin R. and Sharp, Julie A. 2013, Identification and functional characterization of a novel monotreme - specific antibacterial protein expressed during lactation, PLoS one, vol. 8, no. 1, pp. 1-12.

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Title Identification and functional characterization of a novel monotreme - specific antibacterial protein expressed during lactation
Author(s) Bisana, Swathi
Kumar, Satish
Rismiller, Peggy
Nicol, Stewart C.
Lefevre, Christophe
Nicholas, Kevin R.
Sharp, Julie A.
Journal name PLoS one
Volume number 8
Issue number 1
Start page 1
End page 12
Total pages 12
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2013
ISSN 1932-6203
Summary Monotremes are the only oviparous mammals and exhibit a fascinating combination of reptilian and mammalian characters. They represent a component of synapsidal reproduction by laying shelled eggs which are incubated outside the mother’s body. This is accompanied by a prototherian lactation process, marking them as representatives of early mammals. The only extant monotremes are the platypus, and the short- and long- beaked echidnas, and their distributions are limited to Australia and New Guinea. Apart for a short weaning period, milk is the sole source of nutrition and protection for the hatchlings which are altricial and immunologically naive. The duration of lactation in these mammals is prolonged relative to the gestational length and period of incubation of eggs. Much of the development of monotreme young occurs in the non-sterile ex-utero environment. Therefore the role of milk in the growth, development and disease protection of the young is of significant interest. By sequencing the cDNA of cells harvested from monotreme milk, we have identified a novel monotreme- specific transcript, and the corresponding gene was designated as the EchAMP. The expression profile of this gene in various tissues revealed that it is highly expressed in milk cells. The peptides corresponding to the EchAMP protein have been identified in a sample of echidna milk In silico analysis indicated putative antimicrobial potential for the cognate protein of EchAMP. This was further confirmed by in vitro assays using a host of bacteria. Interestingly, EchAMP did not display any activity against a commensal gut floral species. These results support the hypothesis of enhancement of survival of the young by antimicrobial bioactives of mammary gland origin and thus emphasize the protective, non- nutritional role of milk in mammals.
Language eng
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30055427

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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